Antibody-Induced Activation of β1 Integrin Receptors Stimulates cAMP-Dependent Migration of Breast Cells on Laminin-5

Abstract

The β1 integrin-stimulating antibody TS2/16 induces cAMP-dependent migration of MCF-10A breast cells on the extracellular matrix protein laminin-5. TS2/16 stimulates a rise in intracellular cAMP within 20 min after plating. Pertussis toxin, which inhibits both antibody-induced migration and cAMP accumulation, targets the Gαi3 subunit of heterotrimeric G proteins in these cells, suggesting that Gαi3 may link integrin activation and migration via a cAMP signaling pathway.