Antifertility Effects of Seeds of Sapindus trifoliatus on Histology of Testis and Epididymis of Male Albino Mice (Mus Musculus Albinus)

Abstract

The present study was carried out as a preliminary study to determine the histological effects of seed powder of Sapindus trifoliatus on male albino mice. A total of 12 adult male albino mice were taken. Four groups each containing three mice were made. First group of mice served as control and other three groups were treated as experimental. The control mice were fed with normal diet, while experimental mice were administered with 10, 30 and 50 mg/kg body weight seed powder respectively for 30 days. After 30 days of experimentation all control and treated mice were dissected. The testis and epididymis were removed, their weights were recorded, fixed and processed for further histological technique. Administration of the S. trifoliatus to mice showed a significant increase in body weight, but testicular weight were unaffected. The present study also clearly demonstrates that the administration of seed powder of S. trifoliatus at all the tested doses caused insignificant reduction of the reproductive organs weight, testicular damage and complete arrest of spermatogenesis. At lower doses, a few layers of spermatogenic cells were noted but at later stage the spermatogenesis was stopped. At the highest dose i.e. 50 mg complete arrest of spermatogenesis and degeneration of remaining germinal cells could be seen. The atrophy of Leydig’s cells was also noted at higher doses of treatment up to 60 days. The epididymis also showed degenerative changes in the epithelial cells at higher doses. Spermatozoa were not seen in the lumen of ductules of epididymis.