Nanopolymeric substrates for cyto-regulatory gene program interrogation

Abstract

Functionalized block copolymers that possess nanoscale thicknesses represent an important class of biomimetic materials with potential applications in drug delivery, membrane/protein-based devices, as well as cellular interrogation platforms for basic science studies. A key element that serves as the foundation for the translational applicability of this material is represented by the examination of its effects upon cyto-regulatory gene programs that govern processes such as cellular stress and inflammation. With a better understanding of the cellular response to these materials, improved design principles can be examined towards the utilization of these polymers for biomedical applications in vivo. Here we present a comprehensive assessment of the basal levels of secretion for a spectrum of inflammatory cytokines/molecules including tumor necrosis factor-alpha (TNFalpha), interleukin-6 (IL-6), interleukin-12 (IL-12), as well as inducible nitric oxide synthase (iNOS). In addition, we examine the effects of cellular incubation with the triblock copolymer in solution upon morphology as well as growth capabilities in vitro. The foundational information gleaned from this study will provide an important glimpse into the internal cellular response to foreign material contact towards the forging of devices fabricated at the interface of biology and artificial materials.