"Association of Fibroblast Growth Factor Receptor Gene (FGFR2) Polymorphism (rs2981582) and its Expression with Breast Cancer"

Abstract

The association between Fibroblast Growth Factor Receptor 2 (FGFR2) gene polymorphism and expression with breast cancer varies with ethnicity and geographical distribution. Several studies have been conducted with different genotypes of this particular gene polymorphism among various ethnicities throughout the world. The main purpose of this study was to evaluate the relationship between FGFR2 (rs2981582) gene polymorphism and FGFR2 gene expression with breast cancer in Indian women and also to establish the relative gene expression with different genotypes of the polymorphism, if there is any. Method: A total of 75 cases were selected, out of which 25 were malignant cases,25 healthy controls and 25 benign cases. Restriction Fragment Length Polymorphism Polymerase Chain Reaction (RFLP PCR) was used to determine FGFR2 Single Nucleotide Polymorphism and Real Time Quantitative Polymerase Chain Reaction (RT qPCR) for FGFR2 gene expression. The strength of association was estimated using Odds Ratios (ORs) and 95% Confidence Intervals (CIs). RESULTS: Our results showed that FGFR2 (rs2981582) polymorphism is not significantly associated with breast cancer in Indian women. However, FGFR2 gene expression is significantly increased in breast cancer but the differential gene expression associated with different genotypes (TT / TC / CC) is not significant in both malignant and benign cases. Therefore, we conclude from this study that FGFR2 rs2981582 SNP does not influence the expression of FGFR2 in this population.