Impedance biosensor based on interdigitated electrode arrays for detection of low levels of E.coli O157:H7

2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS 2013) : Taipei, Taiwan, 20-24 January 2013. IEEE International Conference on Micro Electro Mechanical Systems (26th : 2013 : Taipei, Taiwan) Pub Date : 2013-03-07 DOI:10.1109/MEMSYS.2013.6474404

S. G. Dastider, Syed Barizuddin, Yifan Wu, M. Dweik, M. Almasri

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引用次数: 11

Abstract

We report an interdigitated electrode array (IDEA) impedance biosensor with rapid detection of Escherichia coli O157:H7. This biosensor has significantly better detection capability compared with the conventional microbiological testing method. The total detection time for this biosensor is under 3 hours. Dielectrophoresis (DEP) is used in conjunction with impedance spectroscopy to improve the detection capability of the biosensor. The IDEA surface is functionalized using anti-E.coli antibody for selectivity of the bacteria. The dose response was in-between 3 × 106 CFU/mL and 3 × 102 CFU/mL. This is 10 fold better than the detection limit previously reported using impedance based biosensors.

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基于交叉指状电极阵列的阻抗生物传感器检测低水平大肠杆菌O157:H7

我们报道了一种能够快速检测大肠杆菌O157:H7的交叉指状电极阵列阻抗生物传感器。与传统的微生物检测方法相比，该传感器具有明显更好的检测能力。该生物传感器的总检测时间在3小时以下。Dielectrophoresis (DEP)与impedance spectroscopy(阻抗谱)结合使用来提高生物传感器的检测能力。IDEA表面使用anti-E功能化。大肠杆菌抗体对细菌的选择性。剂量效应介于3 × 106 CFU/mL和3 × 102 CFU/mL之间。这比以前报道的基于阻抗的生物传感器的检测极限好10倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS 2013) : Taipei, Taiwan, 20-24 January 2013. IEEE International Conference on Micro Electro Mechanical Systems (26th : 2013 : Taipei, Taiwan)

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