Assessment of Semen Quality of Two Ram Breeds at Pre-freeze Stage of Cryopreservation

International Journal of Livestock Research Pub Date : 2021-01-01 DOI:10.5455/IJLR.20201026053211

M. Asaduzzaman, A. Saha, S. Akter, P. Jha, Alam, F. Bari

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引用次数: 1

Abstract

Pre-freeze semen quality is a prediction for an efficient and successful freezing in cryopreservation. This study was designed to assess the semen quality of two ram breeds at pre-freeze stage of cryopreservation. A total of 30 ejaculates (5 ejaculates per ram) were collected using artificial vagina twice a week for a period of two months from two sexually active ram breeds; indigenous (n=3), and Muzaffarnagari crossbred (n=3), and evaluated for semen characteristics. Ejaculate volume, color, mass activity, and sperm concentration were evaluated for fresh semen while motility, viability, morphologically normal sperm, intact membrane, intact acrosome values were evaluated in both fresh (370C) and glycerol equilibrated (50C, pre-freeze) stage. Sperm concentration was found significantly (p<0.05) higher in Muzaffarnagari crossbred rams. Sperm motility, viability, morphologically normal, intact membrane, and intact acrosome values have not differed significantly (p>0.05) at the fresh stage but varied significantly (p<0.05) at pre-freezing stage. Therefore, it can be predicted that the selected breeding rams are of good quality freezer for frozen semen production.

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两个公羊品种冷冻前期精液质量评价

冷冻前精液质量是有效和成功冷冻的预测指标。本研究旨在评估两个品种的公羊在冷冻前阶段的精液质量。从两个性活跃的公羊品种中，每周两次使用人工阴道收集30次射精(每只公羊5次射精)，为期两个月;本地(n=3)和Muzaffarnagari杂交(n=3)，并评估其精液特征。在新鲜(370C)和甘油平衡(50C，预冻)阶段分别评估精液的体积、颜色、质量活性和精子浓度，同时评估活力、活力、形态正常精子、完整膜和完整顶体的价值。鲜育期精子浓度差异显著(p<0.05)，冷冻前精子浓度差异显著(p<0.05)。由此可以预测，所选种羊是生产冷冻精液的优质冷冻机。

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International Journal of Livestock Research

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