Acute and subacute toxicity studies of hydroethanolic extract of Baillonella toxisperma Pierre fruit pulp

Abstract

Baillonella toxisperma Pierre, fruit pulp is largely used in traditional medicine. No toxicity study was done on this fruit pulp. The purpose of this study was to assess the acute and subacute toxicity of the hydroethanolic extract of the fruit pulp of B. toxisperma. An acute toxicity study was conducted at 2000 mg/kg of body weight as a 14-day limit test in Wistar albino rats. Throughout the experiment, general signs of toxicity were noted. Subsequently, a subacute toxicity study was performed at a dose of 400     mg/kg of body weight for 28 days. General signs of toxicity were also noted, after animal sacrifice, histological, hematological, and biochemical analyses (ALAT, ASAT, triglycerides, total cholesterol, total proteins, creatinine) were performed. At LD50?2000 mg/kg, no general signs of toxicity were noted in either acute or subacute conditions. No signs of toxicity were noted histologically, while slight increases in granulocytes and decreases in lymphocytes were noted hematologically. Biochemically, while no renal observations were made, the extract caused hepatic cytolysis. The fruit pulp of B. toxisperma P could be hepatotoxic at high doses. Key words: Hydroethanolic extract of Baillonella toxisperma Pierre fruit pulp, acute toxicity, subacute toxicity.