Improvement of lincomycin production by mutant selection and metabolic regulation

X. Pang, Yitao Zheng, Xianting Qiao, Quangui Mao, Q. Ma, Rui-fang Ye
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引用次数: 5

Abstract

ABSTRACT Lincomycin is a lincosamide antibiotic produced by Streptomyces lincolnensis. Through mutagenesis by ethylmethansulfonate (EMS) and ultraviolet (UV) irradiation repeatedly, M2 was picked out in plate with glutamine and propylproline orderly. In 50-L stirred bioreactor, the production of lincomycin, fermented by M2, was increased to 8136 u/ml under the optimal condition as compared to original strain S. lincolnensis 07–5 (6634 u/ml). Two-dimensional gel electrophoresis (2-D GE) and mass spectrometry (MS)-shown LmbG, LmbI, and acetohydroxy acid isomeroreductase were remarkably synthesized in M2. The gene lmbG and lmbI are responsible for methylation in the lincomycin biosynthetic cluster, while acetohydroxy acid isomeroreductase contributes to stronger metabolic capability. Finally, we obtained a better strain for industrial production.
突变体选择和代谢调控提高林可霉素产量
林可霉素是林肯链霉菌生产的一种林可沙胺类抗生素。通过甲磺酸乙酯(EMS)诱变和紫外线(UV)反复照射,M2在谷氨酰胺和丙氨酸的作用下有序地在平板上被挑出。在50 l的搅拌生物反应器中,M2发酵的林可霉素产量比原菌株S. lincolnensis 07-5 (6634 u/ml)提高到8136 u/ml。二维凝胶电泳(2-D GE)和质谱(MS)显示,M2中显著合成了LmbG、LmbI和乙酰羟基酸异构还原酶。lmbG和lmbI基因在林可霉素生物合成簇中负责甲基化,而乙酰羟基酸异构还原酶则具有更强的代谢能力。最终获得了一种适合工业生产的优良菌株。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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