Localization of glutelin gene in rice chromosome by in situ hybridization

Abstract

The location of storage protein glutelin gene on a chromosome was determined by in situ hybridization made between glutelin cDNA clone and mitotic chromosomes in rice. After hybridization, the location of 3H-labelled glutelin gene was analyzed on chromosomes of twenty one cells. It was found that silver grains were mainly concentrated on the short arm of chromosome 2 (K2 chromosome), demonstrating that the gene responsible for storage protein glutelin was located on this chromosome.