Adsorption of tetracycline antibiotics to gold nanoparticles and feasibility of aptamer-based label-free colorimetric detection

IF 1.1 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Yuheng Song, Derek H. G. von Eppinghoven, Yang Zhou, Hanxiao Zhang, Juewen Liu
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引用次数: 0

Abstract

Tetracyclines are a group of very important antibiotics that are still in use to date. To extract, detect and remove tetracyclines from the environment, various nanomaterials have been employed. Although gold nanoparticles (AuNPs) are a commonly cited material for these purposes, fundamental understanding of these tetracycline-AuNP systems are still limited. In this work, the adsorption of tetracycline, oxytetracycline and doxycycline to AuNPs was studied. The effect on the colloidal stability of AuNPs, adsorption kinetics, and the resulting adsorption isotherms were measured. While millimolar concentrations of the tetracyclines can cause aggregation of AuNPs, saturated monolayer adsorption was achieved with low micromolar concentrations of the tetracyclines. Adsorption was instantaneous, and adsorption to AuNPs enhanced their intrinsic fluorescence instead of quenching. With the assumption of aptamer/target complexes cannot be easily adsorbed by AuNPs compared to free aptamers, a label-free colorimetric detection method was tested. While the label-free sensor showed target-dependent aggregation of AuNPs, a non-binding mutant aptamer showed the same trend, suggesting that the color change did not reflect aptamer adsorption but other events such as target adsorption. This study indicates the importance of fundamental understanding of target/AuNP interactions to correctly design aptamer and AuNP-based label-free biosensors.
四环素类抗生素在金纳米颗粒上的吸附及基于适配体的无标记比色检测的可行性
四环素是迄今仍在使用的一组非常重要的抗生素。为了从环境中提取、检测和去除四环素,各种纳米材料已经被使用。尽管金纳米颗粒(AuNPs)是用于这些目的的常用材料,但对这些四环素- aunp系统的基本理解仍然有限。本文研究了四环素、土霉素和强力霉素在AuNPs上的吸附。测定了其对AuNPs胶体稳定性、吸附动力学以及吸附等温线的影响。虽然毫摩尔浓度的四环素可以引起aunp的聚集,但低微摩尔浓度的四环素可以实现饱和的单层吸附。吸附是瞬时的,对AuNPs的吸附增强了其固有荧光而不是猝灭。假设与游离适配体相比,适配体/靶复合物不易被AuNPs吸附,我们测试了一种无标记比色检测方法。无标签传感器显示AuNPs的目标依赖性聚集,而非结合突变体适体显示相同的趋势,这表明颜色变化不是反映适体吸附,而是反映其他事件,如目标吸附。这项研究表明,对靶标/AuNP相互作用的基本理解对于正确设计适配体和基于AuNP的无标签生物传感器的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Canadian Journal of Chemistry
Canadian Journal of Chemistry 化学-化学综合
CiteScore
1.90
自引率
9.10%
发文量
99
审稿时长
1 months
期刊介绍: Published since 1929, the Canadian Journal of Chemistry reports current research findings in all branches of chemistry. It includes the traditional areas of analytical, inorganic, organic, and physical-theoretical chemistry and newer interdisciplinary areas such as materials science, spectroscopy, chemical physics, and biological, medicinal and environmental chemistry. Articles describing original research are welcomed.
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