Klonierung von RSV‐cDNS (Respiratiorisches Syncytial Virus) und erste Charakterisierung der Rekombinanten

Abstract

Genomic RNA of the Respiratory syntical virus (RS-virus) was used as a template to synthesize calfthymus DNA-primed cDNAs. These cDNAs were subsequently cloned into the E. coli plasmid pBR 322 at the PstI-site. By hybridization 6 recombinants with virusspecific cDNAs representing 10% of the whole genome could be isolated.