The role of plant adaptogen in the correction of parameters of the hemostasis system during ­emotional-immobilization stress in the experiment

Abstract

Background. Maladaptive changes on the part of various organs and systems, in particular on the part of the hemostasis system, are manifested in extreme forms of physical inactivity, one of which is immobilization stress. If the organism is also subjected to psycho-emotional effects at the same time, then the combined effect of stressors is accompanied by an increase in maladaptive disorders. Aim. To establish the influence of emotional-immobilization stress on the hemostasis system, as well as the possibility of correcting changes by taking a course of eleutherococcus. Material and methods. The experiment was conducted on male rats, which were divided into four groups: the first group (n=14) control, the second group (n=14) animals treated with eleutherococcus for 30 days, the third group (n=14) rats, subjected to a single 3-hour water-immersion dipping, the fourth group (n=14) animals that took eleutherococcus for 30 days before the water-immersion dipping. The state of the hemostasis system was assessed in all experimental groups. Statistical processing of the results was performed using the Statistica 10.0 package (Statsoft, USA). Significance of differences was assessed using the nonparametric MannWhitney test and Student's t-test. Results. A single immobilization effect was accompanied by hypercoagulable changes, which characterized by shortening of the silicone (p=0.001) and kaolin (p=0.002) time. Completion of the 30-day adaptogen intake followed by emotional immobilization stress was accompanied by an increase in silicone (p=0.001), kaolin (p=0.021), prothrombin (p=0.021) and thrombin (p=0.006) time, which indicated hypocoagulation changes. Conclusion. The immobilization effect is characterized by hypercoagulability against the background of a decrease in the anticoagulant and fibrinolytic activity of the blood plasma; the course intake of eleutherococcus prior to immobilization is accompanied by hypocoagulant changes and an increase in the anticoagulant activity of the blood plasma.