Microwave-assisted extraction, structural elucidation, and in vitro anti-glioma and immunostimulatory activity of polysaccharide from Panax ginseng C. A. Meyer

IF 5.6 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2022-12-01 DOI:10.1016/j.indcrop.2022.115729

Dongxue Cao , Enwei Wei , Zeyu Wang , Zhengyu Hu , Ling Qi , Hongli Zhou , Jingli Zhao

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引用次数: 2

Abstract

This paper aimed to elaborate the chemical characteristics of ginseng polysaccharide via chemical methods and instrumental analyses; additionally, the anti-glioma activity and immunostimulatory capacity were also evaluated via biological experiments. Ginseng polysaccharide by microwave-assisted extraction (MEGP) method was purified by spongiform diethylaminoethyl cellulose (DEAE) and further separated by DEAE-52 column chromatography to obtain four MEGP fractions (MEGP-0, MEGP-1, MEGP-2, MEGP-3). Utilizing Zeta potential analyzer and X-ray photoelectron spectroscopy (XPS), MEGPs were, for the first time, confirmed the valence state of C and O with the binding mode of elements, and all MEGP fractions contained triple-helix structure. MEGP-2 and MEGP-3 significantly reduced the cell growth rate of TJ905 and U87, showing an inhibitory effect on glioma. Among four fractions, MEGP-2 markedly elevated the immunostimulatory capacity via the activation of MAPK signaling route, with an increasing the expression levels of p-p38, p-ERK and p-JNK. In summary, this paper laid a solid foundation for the development of ginseng, as a promising drug or tonic, in the fields of anti-glioma and immunoregulation.

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人参多糖的微波辅助提取、结构鉴定及体外抗胶质瘤和免疫刺激活性

本文旨在通过化学方法和仪器分析阐述人参多糖的化学特性;此外，还通过生物实验对其抗胶质瘤活性和免疫刺激能力进行了评价。用海绵状二乙胺乙基纤维素(DEAE)纯化微波辅助提取人参多糖(MEGP)，再用DEAE-52柱层析分离得到4个MEGP组分(MEGP-0、MEGP-1、MEGP-2、MEGP-3)。利用Zeta电位分析仪和x射线光电子能谱(XPS)，首次用元素的结合方式证实了C和O的价态，所有MEGP组分均含有三螺旋结构。MEGP-2和MEGP-3显著降低TJ905和U87的细胞生长速率，对胶质瘤有抑制作用。四组分中，MEGP-2通过激活MAPK信号通路显著提高免疫刺激能力，p-p38、p-ERK、p-JNK表达水平升高。综上所述，本研究为人参在抗胶质瘤和免疫调节等领域的发展奠定了坚实的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.