Real-time measurement of fluorescence spectra from single airborne biological particles

Field Analytical Chemistry and Technology Pub Date : 1999-01-01 DOI:10.1002/(SICI)1520-6521(1999)3:4/5<221::AID-FACT2>3.0.CO;2-7

S. C. Hill, R. Pinnick, S. Niles, Yong-le Pan, S. Holler, R. Chang, J. Bottiger, Bean T. Chen, C. Orr, G. Feather

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引用次数: 214

Abstract

Improved real-time methods for characterizing airborne biological particles are needed. Here we review our efforts in developing techniques for measuring the laser-induced fluorescence (total and spectrally dispersed) of individual airborne particles, and describe our present system, which can measure fluorescence spectra of single micrometer-sized bioaerosol particles with good signal-to-noise ratios. We demonstrate the capability of this system by showing measured spectra of a variety of airborne particles generated in the laboratory from road dust, ammonium sulfate, Bacillus subtilis and other bacteria prepared under various conditions, allergens, cigarette smoke, and chicken-house dust. These spectra illustrate the capability of the system to distinguish between some biological and nonbiological aerosols, and among several types of laboratory-generated biological aerosols. We suggest improvements needed to make our system field portable. © 1999 John Wiley & Sons, Inc.* Field Analyt Chem Technol 3: 221–239, 1999

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实时测量单个空气传播生物颗粒的荧光光谱

需要改进的实时方法来表征空气中的生物颗粒。在这里，我们回顾了我们在开发测量单个空气颗粒的激光诱导荧光(总荧光和光谱分散)的技术方面所做的努力，并描述了我们目前的系统，该系统可以测量单个微米大小的生物气溶胶颗粒的荧光光谱，具有良好的信噪比。我们通过展示在实验室中由道路粉尘、硫酸铵、枯草芽孢杆菌和其他在各种条件下制备的细菌、过敏原、香烟烟雾和鸡舍粉尘产生的各种空气传播颗粒的测量光谱来证明该系统的能力。这些光谱说明了该系统区分某些生物和非生物气溶胶以及几种实验室生成的生物气溶胶的能力。我们建议改进，使我们的系统现场便携。©1999 John Wiley & Sons, Inc.* Field analyst Chem technology 3: 221-239, 1999

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Field Analytical Chemistry and Technology

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