Variability in cacao selected by producers for resistance to witchesbroom based on microsatellite markers

IF 1.3 4区农林科学 Q3 AGRONOMY

Crop Breeding and Applied Biotechnology Pub Date : 2004-09-30 DOI:10.12702/1984-7033.V04N03A05

A. S. G. Faleiro, F. Faleiro, U. Lopes, G.R.P. Melo, W. Monteiro, Rita C.S Bahia, R. Corrêa

引用次数: 8

Abstract

This study aimed at a microsatellite marker-based assessment of the genetic variability of 30 accessions of Theobroma cacao selected for resistance to witchesbroom on commercial plantations in the cocoa region of Bahia State and to compare them with traditional sources of resistance to witchesbroom (SCA-6 and IMC-67) and other parents (ICS-1 and SIC-19) used in the breeding program of the Cocoa Research Center (CEPEC). Sixteen pairs of specific primers for microsatellite loci were used, generating 64 alleles. The genetic distance between the analyzed accessions ranged from 0.13 to 0.71 and the heterozygosis levels from 23 to 76%. The cluster analyses grouped 18 accessions (60%) together with SCA-6. Accessions selected on farms for their high levels of resistance to witchesbroom, good agronomic traits, which are genetically distant from SCA-6, have a high potential for the breeding program or even for direct recommendation to farmers.

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生产者基于微卫星标记选择的可可抗枯病变异

本研究旨在以微卫星标记为基础，对巴伊亚州可可区商业种植园选育的30个抗枯病可可材料的遗传变异进行评估，并将其与可可研究中心(CEPEC)育种计划中使用的传统抗枯病来源(SCA-6和IMC-67)和其他亲本(ICS-1和SIC-19)进行比较。使用了16对微卫星位点特异引物，共产生64个等位基因。遗传距离为0.13 ~ 0.71，杂合度为23% ~ 76%。聚类分析将18个条目(60%)与SCA-6一起分组。在农场中选择的材料具有对金帚病的高抗性，良好的农艺性状，与SCA-6的遗传距离较远，具有很高的育种计划潜力，甚至可以直接推荐给农民。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Breeding and Applied Biotechnology AGRONOMY-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.70

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.