{"title":"Comparative Modeling and Molecular Interaction Study for the Management of AMD and CRVO Ocular Disorder","authors":"Srivastava P","doi":"10.23880/oajo-16000263","DOIUrl":null,"url":null,"abstract":"Age Related Macular Degeneration (AMD) and Central Retinal Vein Occlusion (CRVO) are the rare and leading cause of blindness among patients with ocular problem. Many proteins are reported in the progression of these ocular disorders. Proteins which are directly involved in the development of this disorder reported in the literature, their sequence related information retrieved from biological databases. In silico technique was implemented in order to characterize the properties and structures of the proteins using ProtParam. For studying about the potential phosphorylation sites in protein generally NetPhos server was used whereas for denoting the location of signal peptide cleavage sites and their presence the server which is used is SingalP server. For prediction of secondary structure prediction of proteins is done by using SOPMA. The SOSUI server performs the identification of trans-membrane regions. The 3D dimensional structure was modeled using Swiss Model Workspace and Modeller. Ramachandran plot was used to validate the stereochemical properties of the predicted structures because it is a very important step after 3D structure prediction. Docking of screened phytochemicals with selected proteins was performed by AutoDock. Docking study revealed that Curcumin (binding energy: -8.35) and Berberine (binding energy: -7.14) can be used as better therapeutic lead molecule for the cure of CRVO and AMD respectively.","PeriodicalId":91939,"journal":{"name":"Open access journal of ophthalmology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open access journal of ophthalmology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.23880/oajo-16000263","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Age Related Macular Degeneration (AMD) and Central Retinal Vein Occlusion (CRVO) are the rare and leading cause of blindness among patients with ocular problem. Many proteins are reported in the progression of these ocular disorders. Proteins which are directly involved in the development of this disorder reported in the literature, their sequence related information retrieved from biological databases. In silico technique was implemented in order to characterize the properties and structures of the proteins using ProtParam. For studying about the potential phosphorylation sites in protein generally NetPhos server was used whereas for denoting the location of signal peptide cleavage sites and their presence the server which is used is SingalP server. For prediction of secondary structure prediction of proteins is done by using SOPMA. The SOSUI server performs the identification of trans-membrane regions. The 3D dimensional structure was modeled using Swiss Model Workspace and Modeller. Ramachandran plot was used to validate the stereochemical properties of the predicted structures because it is a very important step after 3D structure prediction. Docking of screened phytochemicals with selected proteins was performed by AutoDock. Docking study revealed that Curcumin (binding energy: -8.35) and Berberine (binding energy: -7.14) can be used as better therapeutic lead molecule for the cure of CRVO and AMD respectively.
年龄相关性黄斑变性(AMD)和视网膜中央静脉阻塞(CRVO)是眼病患者中罕见且主要的致盲原因。许多蛋白质在这些眼部疾病的进展中被报道。文献中报道的直接参与该疾病发展的蛋白质,其序列相关信息可从生物数据库中检索。在硅技术的实施,以表征性质和结构的蛋白质使用ProtParam。研究蛋白质中潜在的磷酸化位点一般使用NetPhos服务器,而表示信号肽裂解位点的位置及其存在则使用SingalP服务器。对于蛋白质的二级结构预测,采用SOPMA进行预测。SOSUI服务器执行跨膜区域的识别。利用Swiss Model Workspace和modeleller对三维空间结构进行建模。Ramachandran图是三维结构预测后非常重要的一步,因此采用Ramachandran图对预测结构的立体化学性质进行验证。通过AutoDock将筛选的植物化学物质与选定的蛋白质进行对接。对接研究发现,姜黄素(结合能:-8.35)和小檗碱(结合能:-7.14)分别可作为治疗CRVO和AMD较好的先导分子。