Assay of Brain Endogenous Formaldehyde With 2, 4-Dinitrophenylhydrazine Through UV-HPLC: Assay of Brain Endogenous Formaldehyde With 2, 4-Dinitrophenylhydrazine Through UV-HPLC

IF 0.2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

生物化学与生物物理进展 Pub Date : 2012-01-03 DOI:10.3724/SP.J.1206.2011.00407

S. Tao, Yan-fei Wei, Rong He

{"title":"Assay of Brain Endogenous Formaldehyde With 2, 4-Dinitrophenylhydrazine Through UV-HPLC*: Assay of Brain Endogenous Formaldehyde With 2, 4-Dinitrophenylhydrazine Through UV-HPLC*","authors":"S. Tao, Yan-fei Wei, Rong He","doi":"10.3724/SP.J.1206.2011.00407","DOIUrl":null,"url":null,"abstract":"Chronic impairments of brain caused by accumulation of endogenous formaldehyde as ageing is thought as one of the risk factors for sporadic age-related dementia.Thus,it is necessary to determine the endogenous concentration of formaldehyde in brain.Using 2,4-dinitrophenylhydrazine(2,4-DNPH) in the assay, the concentrations of formaldehyde in parietal lobe,frontal lobe,temporal lobe,occipital lobe,hippocampus, cerebellum and brainstem of porcine brain(n=5) were measured through UV-HPLC.Homogenizing brain tissues with 10% trichloroacetic acid(pH =1.0) not only avoided the disturbance from contaminated protein and other compounds,but also eliminated the step extraction of FA-DNPH derivative(2,4-dinitrophenylhydrazone) and improve the methodological sensitivity(P 0.05).The porcine brain formaldehyde was 75.5～83.4 μmol/kg fresh brain tissues measured by this improved method.Recoveries of spiked formaldehyde at low and high level were 95.96%～102.04% with relative standard deviations less than 10%.This suggests that the modified 2,4-DNPH method is effective enough to measure the endogenous formaldehyde in brain tissues.","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"11 1","pages":"1171-1177"},"PeriodicalIF":0.2000,"publicationDate":"2012-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"26","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"生物化学与生物物理进展","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3724/SP.J.1206.2011.00407","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 26

Abstract

Chronic impairments of brain caused by accumulation of endogenous formaldehyde as ageing is thought as one of the risk factors for sporadic age-related dementia.Thus,it is necessary to determine the endogenous concentration of formaldehyde in brain.Using 2,4-dinitrophenylhydrazine(2,4-DNPH) in the assay, the concentrations of formaldehyde in parietal lobe,frontal lobe,temporal lobe,occipital lobe,hippocampus, cerebellum and brainstem of porcine brain(n=5) were measured through UV-HPLC.Homogenizing brain tissues with 10% trichloroacetic acid(pH =1.0) not only avoided the disturbance from contaminated protein and other compounds,but also eliminated the step extraction of FA-DNPH derivative(2,4-dinitrophenylhydrazone) and improve the methodological sensitivity(P 0.05).The porcine brain formaldehyde was 75.5～83.4 μmol/kg fresh brain tissues measured by this improved method.Recoveries of spiked formaldehyde at low and high level were 95.96%～102.04% with relative standard deviations less than 10%.This suggests that the modified 2,4-DNPH method is effective enough to measure the endogenous formaldehyde in brain tissues.

查看原文本刊更多论文

用紫外高效液相色谱法测定2,4 -二硝基苯肼脑内甲醛*:用紫外高效液相色谱法测定2,4 -二硝基苯肼脑内甲醛*

随着年龄的增长，内源性甲醛积累引起的慢性脑损伤被认为是散发性年龄相关性痴呆的危险因素之一。因此，有必要测定甲醛在脑内的内源性浓度。以2,4-二硝基苯肼(2,4- dnph)为检测材料，采用紫外高效液相色谱法(UV-HPLC)测定5只猪大脑顶叶、额叶、颞叶、枕叶、海马、小脑和脑干的甲醛浓度。用10%三氯乙酸(pH =1.0)匀浆脑组织，不仅避免了受污染蛋白质等化合物的干扰，而且消除了FA-DNPH衍生物(2,4-二硝基苯腙)的分步提取，提高了方法的灵敏度(P < 0.05)。采用改进方法测得的猪脑甲醛含量为75.5~83.4 μmol/kg。低、高水平加标甲醛回收率为95.96%~102.04%，相对标准偏差小于10%。这表明改进的2,4- dnph方法可以有效地测量脑组织中内源性甲醛。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

生物化学与生物物理进展生物-生化与分子生物学

CiteScore

0.50

自引率

33.30%

发文量

6805

审稿时长

4 months

期刊介绍： Progress in Biochemistry and Biophysics is a monthly, peer-reviewed journal sponsored by Institute of Biophysics, The Chinese Academy of Sciences together with Biophysical Society of China. It started publication in 1974. It mainly reports on the latest developments in biochemistry, biophysics, molecular biology and neuroscience both in China and abroad. It contains several columns such as "reviews and monographs", "rapid and short communications", "research papers", "techniques and methods" and "academic discussions".