Rrelationship between endothelial function, thickness of "intima media" with some clinical and metabolic parameters in hypertensive patients depending on genetic predictors

Abstract

Objective. Тo study the relationship between endothelial function, "intima-media" thickness (IMT) of carotid arteries, some anthropometric, clinical and metabolic parameters in patients with essential arterial hypertension (EAH) depending on the genes polymorphism NOS3 (rs2070744) and GNB3 (rs5443).Material and methods. 100 patients with EAH and 48 practically healthy subjects (control group) participated in the study. All participants underwent clinical and laboratory examinations. The correlation values of Endothelium-Dependent Flow-Mediated Dilation of the Brachial Artery (FMD BA), total nitric oxide metabolites (NO), Soluble Vascular Cell Adhesion Molecule (sVCAM-1), the transcriptional activity of NOS3 gene, and carotid IMT were studied. GNB3 (rs5443) and NOS3 (rs2070744) genotyping were performed by Real-Time PCR. Results. In EAH patients with dominant T-allele carriers of the NOS3 gene (rs2070744) and/or C-allele of the GNB3 gene (rs5443) FMD BA inversely correlates with the triglycerides level (r =-0.45; p=0.042) and carotid IMT (r=-0.88-/-0.44/; P≤0.006-0.001). In the carriers of mutation C-allele of the NOS3 gene and TT-genotype of the GNB3 gene – with sVCAM-1 (r=-0.41; p=0.009), atherogenic index (r=-0.69; p=0.013) and diastolic arterial pressure (r=-0.84; p=0.036).Conclusion. Matrix correlations of endothelial function, IMT with clinical and metabolic parameters in EAH patients showed the dependence of connections on the allelic state of the genes NOS3 (rs2070744) and GNB3 (rs5443).