Ethyl acetate extract of Clausena execavatapromotes growth inhibition of Burkitt’s lymphoma cell line via apoptotic activities

IF 0.7 4区医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas Pub Date : 2023-01-01 DOI:10.37360/blacpma.23.22.3.26

S. Albaayit

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引用次数: 1

Abstract

Clausena excavatais a famous folklore medicinal plant in Asian region that is being used for the treatment of different disorders. This study investigated the cytotoxic effects of leaf extracts via MTT assay, as well as the in vitroapoptotic activities of the ethyl acetate C. excavataleaf extract (EACE) on human Burkitt’s lymphoma, Raji, cell line using annexin-V-FITC/propidium iodide flow cytometric assays. Pro-apoptotic (BAX) and anti-apoptotic (BCL-2, c-MYC) gene expressions were determined via real-time quantitative PCR. Phytochemical screening was done by Gas chromatography-mass spectrometry (GC-MS). EACE has the lowest IC50(19.3 ± 0.35 μg/mL) among extracts. EACE-treated Raji cells after 48 h underwent apoptosis as evident by loss of cell viability and increase in the percentage of early and late apoptotic cells. The results also showed EACE mediated decreased in the BCL-2 and c-MYC gene expressions and increased in the BAX gene. C. excavatais a potential treatment for Burkitt lymphomathrough activation of apoptosis

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克氏菌乙酸乙酯提取物对伯基特淋巴瘤细胞株的生长抑制作用是通过细胞凋亡活性来实现的

黄花是亚洲地区著名的民间药用植物，被用于治疗各种疾病。本研究采用MTT法研究了叶提取物的细胞毒作用，并采用annexin-V-FITC/碘化丙啶流式细胞术研究了乙酸乙酯提取物(EACE)对人Burkitt淋巴瘤Raji细胞株的体外凋亡活性。通过实时定量PCR检测促凋亡(BAX)和抗凋亡(BCL-2、c-MYC)基因的表达。采用气相色谱-质谱联用技术进行植物化学筛选。EACE的IC50最低(19.3±0.35 μg/mL)。eace处理48 h后Raji细胞发生凋亡，细胞活力下降，早期和晚期凋亡细胞比例增加。EACE介导的BCL-2和c-MYC基因表达减少，BAX基因表达增加。C.挖掘是一个潜在的治疗伯基特淋巴瘤通过激活细胞凋亡

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来源期刊

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas PHARMACOLOGY & PHARMACY-

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas (BLACPMA), [Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants]; currently edited by the publishing house MS-Editions, is a bi-monthly international publication that publishes original peerreviewed research in the field of medicinal and aromatic plants, with nearly 20 years of experience. BLACPMA is a scientific journal that publishes two types of articles: Reviews (only in English) and Original Articles (Spanish or English), its main lines of action being agronomy, anthropology and ethnobotany, industrial applications, botany, quality and standardization, ecology and biodiversity, pharmacology, phytochemistry, pharmacognosy, regulatory and legislative aspects. While all areas of medicinal plants are welcome and the experimental approaches used can be broad and interdisciplinary; other areas of research that are not mentioned depend on the Editorial Committee for their acceptance.