Distribution of hydrolytic enzymes at barley powdery mildew encounter sites: implications for resistance associated with papilla formation in a compatible system

Kenji Takahashi , James R. Aist, Herbert W. Israel
{"title":"Distribution of hydrolytic enzymes at barley powdery mildew encounter sites: implications for resistance associated with papilla formation in a compatible system","authors":"Kenji Takahashi ,&nbsp;James R. Aist,&nbsp;Herbert W. Israel","doi":"10.1016/0048-4059(85)90065-7","DOIUrl":null,"url":null,"abstract":"<div><p>Distributions of the hydrolytic enzymes acid phosphatase and non-specific esterase in compatible barley coleoptiles inoculated with <em>Erysiphe graminis</em> fsp. <em>hordei</em> were investigated histochemically by light and electron microscopy. The four different enzymes investigated-acid β-glycerophos-phatase, acid naphthol AS-TR phosphatase, indoxyl acetate esterase and naphthol AS-D acetate esterase—had similar localization patterns. Reaction products were distributed generally in the fungal and host cells, but distinct accumulations were found in certain regions and structures at the encounter sites, including appressoria, penetration pegs, haustoria, papillae, and host cytoplasm. Most papillae showed conspicuous amounts of reaction products, suggesting that papilla formation includes depositions from secretory vesicles derived from the lysosomal system in host cells. There was a marked difference in the apparent concentration of hydrolytic enzyme activity between normal-size papillae accompanied by haustoria (sites of successful penetration) and oversize papillae (sites of unsuccessful penetration). Hydrolytic enzymes, localized most intensively in a lAyer of the oversize papillae, may confer resistance to fungal ingress by degrading components of the penetration pegs.</p></div>","PeriodicalId":101028,"journal":{"name":"Physiological Plant Pathology","volume":"27 2","pages":"Pages 167-184"},"PeriodicalIF":0.0000,"publicationDate":"1985-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0048-4059(85)90065-7","citationCount":"32","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiological Plant Pathology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0048405985900657","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 32

Abstract

Distributions of the hydrolytic enzymes acid phosphatase and non-specific esterase in compatible barley coleoptiles inoculated with Erysiphe graminis fsp. hordei were investigated histochemically by light and electron microscopy. The four different enzymes investigated-acid β-glycerophos-phatase, acid naphthol AS-TR phosphatase, indoxyl acetate esterase and naphthol AS-D acetate esterase—had similar localization patterns. Reaction products were distributed generally in the fungal and host cells, but distinct accumulations were found in certain regions and structures at the encounter sites, including appressoria, penetration pegs, haustoria, papillae, and host cytoplasm. Most papillae showed conspicuous amounts of reaction products, suggesting that papilla formation includes depositions from secretory vesicles derived from the lysosomal system in host cells. There was a marked difference in the apparent concentration of hydrolytic enzyme activity between normal-size papillae accompanied by haustoria (sites of successful penetration) and oversize papillae (sites of unsuccessful penetration). Hydrolytic enzymes, localized most intensively in a lAyer of the oversize papillae, may confer resistance to fungal ingress by degrading components of the penetration pegs.

水解酶在大麦白粉病发生地点的分布:在一个兼容系统中与乳突形成相关的抗性的含义
大麦胚芽组织中水解酶、酸性磷酸酶和非特异性酯酶的分布。用光镜和电镜对Hordei进行组织化学研究。酸性β-甘油磷酸酶、酸性萘酚AS-TR磷酸酶、吲哚酚乙酸酯酶和萘酚AS-D乙酸酯酶具有相似的定位模式。反应产物一般分布在真菌和宿主细胞中,但在接触部位的某些区域和结构中发现了明显的积累,包括附着胞、穿透钉、吸器、乳头和宿主细胞质。大多数乳突显示出大量的反应产物,表明乳突的形成包括来自宿主细胞溶酶体系统的分泌囊泡的沉积。正常大小乳头伴吸器(成功渗透部位)与超大乳头伴吸器(不成功渗透部位)的水解酶活性表观浓度有显著差异。水解酶,最密集地定位在一个层的超大乳头,可能赋予抵抗真菌的入侵通过降解的成分穿透钉。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信