Neuropeptide Galanin Increase ROS and IL-1? Production by Blood Cells from Patients with Multiple Sclerosis

Abstract

Objective: Galanin (GAL), a neuropeptide produced in the central and peripheral nervous system, has been involved in the modulation of the inflammatory response. Our present study aimed to evaluate the effect of GAL on the production of reactive oxygen species (ROS) by granulocytes and the secretion of IL-1β by peripheral blood mononuclear cells (PBMNC) from patients with Multiple Sclerosis (MS) and healthy control cells, comparatively.Methods: Granulocytes and PBMNC from patients with MS and healthy controls were purified simultaneously by the Ficoll-Hypaque gradient method. The effect of GAL on ROS generation by granulocytes was performed in a luminol-dependent chemiluminescence assay. The quantification of IL-1β in the supernatant of cultured-PBMNC was determined using immunoassay (ELISA).Results: Our results showed that ROS production in the presence of GAL was significantly higher (p 0.05). Conclusion: GAL modulates the production of ROS and activates inflammasome evaluated by the increase of IL-1β secretion and it may have consequences in the inflammatory process observed in MS.