Rapid determination of bacteria in drinking water using an ATP assay

R. A. Deininger, Jiyoung Lee
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引用次数: 78

Abstract

The presently used heterotrophic plate count (HPC) for the evaluation of the total number of bacteria in a sample of drinking water takes 7 days of incubation. When the results are known, the water has been consumed and is ineffective for the protection of the health of the consumers. Operators of water treatment systems need to know the bacterial water quality in near real time. Contamination of the system, whether it is intentional, accidental, or due to an inadequate disinfectant residual needs to be discovered much sooner because intervention can then take place in the form of flushing low quality water and/or raising the disinfectant residual. The purpose of this study was therefore to determine if a rapid ATP assay can estimate the HPC in minutes. Two additional methods were used for some samples. The first method was the acridine orange direct count (AODC) that enumerates both viable and nonviable bacteria. The second method was the direct viable count (DVC) that enumerates only viable bacteria. Water samples were obtained from local, national, and international locations. The sample selection criteria were based on proximity to the laboratory, cooperating water utilities, and the travel of the authors. The results of the study show that the rapid ATP assay is highly correlated with the conventional plate count method and the DVC method. The significance of the ATP assay is that it can determine the bacterial quality of the drinking water in less than 5 min. © 2001 John Wiley & Sons, Inc. Field Analyt Chem Technol 5: 185–189, 2001
使用ATP试验快速测定饮用水中的细菌
目前用于评估饮用水样品中细菌总数的异养平板计数(HPC)需要7天的孵育。当结果被知道时,水已经被消耗,并且对保护消费者的健康无效。水处理系统的操作人员需要近乎实时地了解细菌水质。系统的污染,无论是故意的,意外的,还是由于消毒剂残留不足,都需要尽快发现,因为可以通过冲洗低质量水和/或提高消毒剂残留的形式进行干预。因此,本研究的目的是确定快速ATP测定是否可以在几分钟内估计HPC。对一些样品使用了另外两种方法。第一种方法是吖啶橙直接计数法(AODC),该方法同时枚举活菌和非活菌。第二种方法是直接活菌计数(DVC),它只枚举活菌。从当地、全国和国际地点获得水样。样本的选择标准是基于靠近实验室、合作的自来水公司和作者的旅行。研究结果表明,ATP快速测定与常规平板计数法和DVC法高度相关。ATP试验的意义在于它可以在5分钟内确定饮用水中的细菌质量。©2001 John Wiley & Sons, Inc。化学工程学报(英文版),2001
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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