A Study on Media Optimization, Characterization and Purification of L-Asparaginase Producing Streptomyces sp. FSOF Strain Isolated from the Bay of Bengal Coastal Line

Abstract

The vast marine biodiversity provides abundant opportunities for the isolation and characterization of Streptomyces species with therapeutic applications. The present study is focused on isolation, characterization, media optimization of Streptomyces sp. FSOF strain for production and purification of L-Asparaginase at an economic level. Methods: The isolated strain from the intertidal region of Bay of Bengal coastal region is characterized by physicochemical, genotypic and by phylogenetic methods. The growth medium of Streptomyces FSOF strain is optimized for high enzyme activity and further purified by Ion exchange chromatography. Results and Conclusion: The FSOF isolate exhibited 4.3 IU/ml enzyme activity in m9 broth. The enzyme activity of the FSOF strain is further analyzed in various growth mediums where 5.9 IU/ml activity is observed in tryptic soy broth. The purified L-asparaginase from FSOF isolate exhibited 23.33 IU/mg specific activity with 3.12 fold purification. The 16s rRNA sequence of FSOF isolate is found to have 99.88% similarity with Streptomyces albidoflavius and Streptomyces odorifer strains. The sequence is further deposited in GenBank (accession number MN562193.1) for future analysis. © 2020 iGlobal Research and Publishing Foundation. All rights reserved.