Antibacterial Activities of Staphylococcus sciuri APD7 Isolate Sponge-Associated Aplysina sp. Against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus

Abstract

Sponges including multicellular animals, do not have actual tissues and organs, and are the simplest animals structurally. Sponges are also a group of marine life that is symbiotic with the microbial community. Microbes that have formed a symbiotic relationship with a sponge will produce secondary metabolites which also protect their host from pathogens. The sponge used in this study is the Aplysina sp. sponge collected from Enggano island, Bengkulu Province. Aplysina sp sponge is known to contain metabolites with antimicrobial, repellent properties, fighting predators, cytotoxic activity of breast cancer cells, has an antibiotic and anti-inflammatory effect on gram-positive bacteria. This study aims to identify of potential isolates associated with Aplysina sp. sponge collected from Enggano island. Isolation of bacteria from Aplysina sp. sponge using Sea Water Complete (SWC) media. The isolates were screened by antagonistic test, morphological characters, Gram-staining, biochemical test and molecular identification. Based on the antagonistic test, APD7 isolate could inhibit Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus in vitro. APD7 isolate was identified molecularly using of 16S rRNA genes analysis and it genetically close with Staphylococcus sciuri.