Biohybrid surface preparation for protein/DNA microarray applications

Abstract

The chemistry of slide substrates used in microarray technology is important, since it determines the spot morphology, signal intensity, low carry over and low evaporation rate. In this paper the experiments developed one substrate type - porous silicon as base for micro arrays with a gold layer film deposit on top. It was study two gold texture in order to find the best report between signal intensity and spot morphology. The experiments reveal that: (i) porous silicon substrate for both functionalization methods assure spots uniformity and a good relationship between signal intensity and solution concentration; (ii) it was observed bonds saturation for high concentrations for both types of samples.