Effect of Replacing Bone Ash with Fresh Water Snail (Pila Ampullacea) Shell Ash on Serum Biochemical Indices of Weaned Rabbits

Abstract

Twenty five (25) mixed breed weaned rabbits were utilized in 84-days experiment to investigate the effect of replacing bone ash with fresh water snail (Pila ampullacea) shell ash on the serum biochemical composition of weaned rabbits. The experiment was conducted at the Teaching and Research Farm, University of Agriculture, Makurdi, Benue State, Nigeria for a period of 12 weeks. The rabbits were randomly assigned to the five dietary treatments and replicated five times giving a total of one rabbit per replicate in a completely randomized design (CRD).   Rabbits were raised intensively in hutches of about 4ft × 4ft. Five experimental diets tagged T1 to T5 were formulated  such that fresh water snail (Pila ampullacea) shell ash replaced bone ash at 0%, 25%, 50%, 75% and 100%. Feed and water were served ad libitum. Bio sanitary and bio security measures were strictly adhered to. At the end of the feeding trial, three rabbits per treatment were selected for the evaluation of serum biochemistry. The parameters assayed for were: total serum protein, serum globulin, serum albumin, cholesterol, serum calcium, and serum phosphorus. The study showed that the serum biochemical indices were not influenced (P˃0.05) by the dietary treatments except for calcium which was significantly (P<0.05) influenced, however, calcium values were within the normal reference values for rabbits. This study had shown that fresh water snail (Pila ampullacea) shell ash can serve as a substitute for bone ash in weaned rabbit diet up to 100% inclusion levels without adverse effect on the serum biochemical indices.