Bismuth Oxide Nanoparticles Induced Oxidative Stress-Related Inflammation in SH-SY5Y Cell Line

E. Oztas, Mahmoud Abudayyak, Beyza Aykanat, Zubeyde Can, E. Baram, G. Özhan
{"title":"Bismuth Oxide Nanoparticles Induced Oxidative Stress-Related Inflammation in SH-SY5Y Cell Line","authors":"E. Oztas, Mahmoud Abudayyak, Beyza Aykanat, Zubeyde Can, E. Baram, G. Özhan","doi":"10.26650/istanbuljpharm.2019.19020","DOIUrl":null,"url":null,"abstract":"DOI : 10.26650/IstanbulJPharm.2019.19020 Bismuth (III) oxide nanoparticles’ (Bi2O3-NPs) unique physicochemical properties attracted the attention in biological, industrial, technological and medical fields. Concurrently, increasing numbers of studies revealing their potential toxic effects and possible toxicity mechanisms are ongoing. In this study, we assessed the toxic potentials of Bi2O3-NPs in human SH-SY5Y neuroblastoma cell line. After Bi2O3-NPs characterization using TEM, the cytotoxic potentials were evaluated by MTT and LDH assays. The induction of reactive oxygen species production was evaluated by H2DCFDA. In order to evaluate the oxidative damages, the changes in antioxidant catalase and superoxide dismutase and glutathione levels were determined. The cellular death pathway and the role of immune response were studied by measuring the mRNA expression levels of related genes. Our results showed that Bi2O3-NPs decreased the cell viability through disruption on mitochondrial activity (IC50:77.57 μg/mL) and membrane integrity (LDH%50:16.97 μg/mL). At 50 μg/mL Bi2O3-NPs, the production of reactive oxygen species (ROS) was induced significantly as well as the catalase and superoxide dismutase levels. In immune response, the mRNA expression levels of interleukin (IL)-6 were increased more than 1.5-fold in all doses; whereas, TNF-α, NF-ĸB and MAPK8 expressions were remained unchanged. Consequently, Bi2O3-NPs induced oxidative stress-related inflammation via activation of pro-inflammatory cytokine, IL-6. You may cite this article as : Oztas E, Abudayyak M, Aykanat B, Can Z, Baram E, Ozhan G (2019). Bismuth Oxide Nanoparticles Induced Oxidative Stress-Related Inflammation in SH-SY5Y Cell Line. Istanbul J Pharm 10.26650/IstanbulJPharm.2019.19020.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"İstanbul Journal of Pharmacy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26650/istanbuljpharm.2019.19020","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

Abstract

DOI : 10.26650/IstanbulJPharm.2019.19020 Bismuth (III) oxide nanoparticles’ (Bi2O3-NPs) unique physicochemical properties attracted the attention in biological, industrial, technological and medical fields. Concurrently, increasing numbers of studies revealing their potential toxic effects and possible toxicity mechanisms are ongoing. In this study, we assessed the toxic potentials of Bi2O3-NPs in human SH-SY5Y neuroblastoma cell line. After Bi2O3-NPs characterization using TEM, the cytotoxic potentials were evaluated by MTT and LDH assays. The induction of reactive oxygen species production was evaluated by H2DCFDA. In order to evaluate the oxidative damages, the changes in antioxidant catalase and superoxide dismutase and glutathione levels were determined. The cellular death pathway and the role of immune response were studied by measuring the mRNA expression levels of related genes. Our results showed that Bi2O3-NPs decreased the cell viability through disruption on mitochondrial activity (IC50:77.57 μg/mL) and membrane integrity (LDH%50:16.97 μg/mL). At 50 μg/mL Bi2O3-NPs, the production of reactive oxygen species (ROS) was induced significantly as well as the catalase and superoxide dismutase levels. In immune response, the mRNA expression levels of interleukin (IL)-6 were increased more than 1.5-fold in all doses; whereas, TNF-α, NF-ĸB and MAPK8 expressions were remained unchanged. Consequently, Bi2O3-NPs induced oxidative stress-related inflammation via activation of pro-inflammatory cytokine, IL-6. You may cite this article as : Oztas E, Abudayyak M, Aykanat B, Can Z, Baram E, Ozhan G (2019). Bismuth Oxide Nanoparticles Induced Oxidative Stress-Related Inflammation in SH-SY5Y Cell Line. Istanbul J Pharm 10.26650/IstanbulJPharm.2019.19020.
氧化铋纳米颗粒诱导SH-SY5Y细胞氧化应激相关炎症
铋(III)氧化物纳米粒子(Bi2O3-NPs)独特的物理化学性质引起了生物、工业、技术和医疗领域的关注。同时,越来越多的研究正在揭示其潜在的毒性作用和可能的毒性机制。在这项研究中,我们评估了Bi2O3-NPs对人SH-SY5Y神经母细胞瘤细胞系的毒性潜能。在用TEM表征Bi2O3-NPs后,用MTT和LDH测定细胞毒性电位。用H2DCFDA评价其对活性氧生成的诱导作用。为了评价氧化损伤,测定了抗氧化过氧化氢酶、超氧化物歧化酶和谷胱甘肽水平的变化。通过检测相关基因mRNA表达水平,研究细胞死亡途径及其在免疫应答中的作用。结果表明,Bi2O3-NPs通过破坏线粒体活性(IC50:77.57 μg/mL)和膜完整性(LDH%50:16.97 μg/mL)来降低细胞活力。50 μg/mL Bi2O3-NPs显著诱导活性氧(ROS)生成,过氧化氢酶和超氧化物歧化酶水平显著升高。在免疫应答中,各剂量组白细胞介素(IL)-6 mRNA表达水平均升高1.5倍以上;而TNF-α、NF-ĸB和MAPK8的表达则保持不变。因此,Bi2O3-NPs通过激活促炎细胞因子IL-6诱导氧化应激相关炎症。你可以将这篇文章引用为:Oztas E, Abudayyak M, Aykanat B, Can Z, Baram E, Ozhan G(2019)。氧化铋纳米颗粒诱导SH-SY5Y细胞氧化应激相关炎症IstanbulJPharm 10.26650/ istanbuljpharma .2019.19020。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信