Analysis of Total Phenolics and Flavonoid from the Root Bark of Flacourtia rukam

Abstract

Flacourtia rukam have been used in the to folk medicine. The roots was used by women after giving birth as antiseptic and the stem bark was used to antihypertensive in the Regency of Musi Banyu Asin South Sumatera Indonesia. Total phenolic and flavonoid content were determined for methanol, ethyl acetate and n-hexane extracts from the root bark of F. rukam. Each extracts were determined antioxidant activity using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) method. The results of the study showed that phenolic higher in methanol extract (71.8868 mg GAE/g) compared with ethyl acetate extract (56.6094 mg GAE/g), while nhexane extract no detection. For flavonoids content showed, methanol extract (2.3116 mg QE/g) and ethyl acetate extract (2.3304 mg QE/g) where the flavonoid content is not significantly different, while n-hexane extract only contained flavonoid 0.8129 mg QE/g. The antioxidant activity the methanol, and ethyl acetate extracts showed IC50 52.91μg/mL and 86.92 μg/mL and in categorized as active antioxidant, while n-hexane extract obtained IC50 values 268.02 μg/ml in category weak antioxidant.