A novel bioreactor for the dynamic flexural stimulation of tissue engineered heart valve biomaterials

Abstract

A novel bioreactor was developed for the purpose of studying the effect of dynamic flexural stimulation on the properties of tissue engineered heart valve (TEHV) scaffolds and constructs. While pulse duplicator and flow loop bioreactors have shown promise in the development of functional tissue engineered cardiovascular constructs, these devices present several drawbacks when applied to the study of fundamental biomechanical phenomena, including: small sample capacity, anatomical sample geometry, and coupled mechanical stimuli. In contrast, our bioreactor was designed to provide a simple, user-controllable mode of mechanical stimulation; cyclic three-point bending; offer a sufficient sample capacity for statistically significant comparisons at multiple time points, and accommodate a simple sample geometry amenable to mechanical testing. The bioreactor has the capacity to dynamically flex twelve rectangular samples (2.5 /spl times/ 0.75 /spl times/ 0.2 cm) under sterile conditions in a humidified cell culture incubator operating at 37/spl deg/C and 5 % CO/sub 2/.