Yeast conversion of mango waste or methanol to single cell protein and other metabolites

Mona M. Rashad, Sorial A. Moharib, Etidal W. Jwanny
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引用次数: 26

Abstract

The utilization of methanol or mango-waste hydrolyzate by Pichia pinus was studied with respect to the biomass, single cell protein and other metabolites. Optimum cultural conditions on both substrates were 30°C and pH 4·8–5. The maximum yield was obtained at the third day of growth (3·6 g/litre in methanol medium, 6·2 g/litre in mango-peel extract medium). At the end of the logarithmic phase, methanol yeast cells contained 52·2% crude protein, 36% true protein and 14·2% nucleic acids, while cells grown on mango-peel extract contained 62·2% crude protein, 39% true protein and 12·9% nucleic acids. The amino acid composition of the protein, especially of the essential acids, was comparable to FAO standards. α-Ketoglutaric and pyruvic acids were found in cells from both substrates but dihydroxyacetone was found in high quantity in methanol medium (976·9 μg%) while acetoacetic acid was found only in mango-peel medium (244·4 μg%).

酵母将芒果废料或甲醇转化为单细胞蛋白等代谢产物
从生物量、单细胞蛋白和其他代谢产物等方面研究了毕赤松木对甲醇和芒果废水解产物的利用。两种基质的最佳培养条件均为30℃,pH值为4·8-5。生长第3天产量最高(甲醇培养基3.6 g/l,芒果皮提取物培养基6.2 g/l)。对数期末,甲醇酵母细胞粗蛋白含量为52.2%,真蛋白含量为36%,核酸含量为14.2%;芒果皮提取物细胞粗蛋白含量为62.2%,真蛋白含量为39%,核酸含量为12.9%。蛋白质的氨基酸组成,特别是必需酸的组成,与粮农组织的标准相当。α-酮戊二酸和丙酮酸在两种基质的细胞中均有检出,但在甲醇培养基中检出大量的二羟基丙酮(976·9 μg%),而乙酰乙酸仅在芒果皮培养基中检出(244·4 μg%)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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