Mona M. Rashad, Sorial A. Moharib, Etidal W. Jwanny
{"title":"Yeast conversion of mango waste or methanol to single cell protein and other metabolites","authors":"Mona M. Rashad, Sorial A. Moharib, Etidal W. Jwanny","doi":"10.1016/0269-7483(90)90059-2","DOIUrl":null,"url":null,"abstract":"<div><p>The utilization of methanol or mango-waste hydrolyzate by <em>Pichia pinus</em> was studied with respect to the biomass, single cell protein and other metabolites. Optimum cultural conditions on both substrates were 30°C and pH 4·8–5. The maximum yield was obtained at the third day of growth (3·6 g/litre in methanol medium, 6·2 g/litre in mango-peel extract medium). At the end of the logarithmic phase, methanol yeast cells contained 52·2% crude protein, 36% true protein and 14·2% nucleic acids, while cells grown on mango-peel extract contained 62·2% crude protein, 39% true protein and 12·9% nucleic acids. The amino acid composition of the protein, especially of the essential acids, was comparable to FAO standards. α-Ketoglutaric and pyruvic acids were found in cells from both substrates but dihydroxyacetone was found in high quantity in methanol medium (976·9 μg%) while acetoacetic acid was found only in mango-peel medium (244·4 μg%).</p></div>","PeriodicalId":100177,"journal":{"name":"Biological Wastes","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0269-7483(90)90059-2","citationCount":"26","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Wastes","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0269748390900592","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 26
Abstract
The utilization of methanol or mango-waste hydrolyzate by Pichia pinus was studied with respect to the biomass, single cell protein and other metabolites. Optimum cultural conditions on both substrates were 30°C and pH 4·8–5. The maximum yield was obtained at the third day of growth (3·6 g/litre in methanol medium, 6·2 g/litre in mango-peel extract medium). At the end of the logarithmic phase, methanol yeast cells contained 52·2% crude protein, 36% true protein and 14·2% nucleic acids, while cells grown on mango-peel extract contained 62·2% crude protein, 39% true protein and 12·9% nucleic acids. The amino acid composition of the protein, especially of the essential acids, was comparable to FAO standards. α-Ketoglutaric and pyruvic acids were found in cells from both substrates but dihydroxyacetone was found in high quantity in methanol medium (976·9 μg%) while acetoacetic acid was found only in mango-peel medium (244·4 μg%).