Pestalotiopsis desseminata PADA JAMBU MENTE BIOLOGI DAN INTERAKSINYA DENGAN Helopeltis antonii

E. M. Adhi, S. Supriadi, S. Rahayuningsih, D. Kilin, N. Karyani
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Abstract

Pestalotiopsis desseminata on cashew: its biology and interaction with Helopeltis antoniiPestalotiopsis desseminata is one of pathogens causing leaf spots. die-back of shoots and inflorescence of cashew plant. It is assumed there is an interaction between the attack of Helopeltis antonii and the fungus in Ihe ield so that the damage becomes more devastating. The objectives of (his research were to investigate several aspects of fungal biology (pathogenicity, mycelial growth, acervuli production, sensitivity to fungicides) and its interaction with //. antonii. The esearch was carried out from April 1999 to March 2000 at the laboratory and green house of Pest and Disease Department Research Institute for Spice and Medicinal Crops, Bogor. /' desseminata was diectly isolated from cashew leaves. Pathogenicity test of P. desseminata isolate was conducted on cashew seedlings. The fungal growth and fungicide effects were carried out in vitro on agar medium. The interaction between P. desseminata and H. antonii was examined by inoculating the fungus and insect, either individually or in combination, on cashew seedlings. The results showed that P. desseminata isolate fomis black spherical acervuli containing oblongs conidia with 5 cells, and the outermost cell has 3 flagellate. Acervuli could only be produced on the cultue illuminated continuously by 600 lux translucent lamp. Pathogenicity test of several isolates of /' desseminata on cashew seedlings caused spherical leaf spot symptom, individually or coalesced as bigger leaf spots. In vitro fungal mycelia growth could be suppressed by several fungicides, including bcnomyl 50% (I ppm) and Uiiopanate-mcthyl 70% (10 ppm) Combining inoculation between P. desseminata and //. antonii resulted in moe severe (devastating) die-back compared with individually inoculated.
腰果上的拟盘多毛孢及其与antonopeltis的相互作用拟盘多毛孢是引起腰果叶斑病的病原菌之一。腰果植物茎部和花序的枯背。据推测,安东尼Helopeltis antonii的攻击与田间真菌之间存在相互作用,因此损害变得更具破坏性。他的研究目的是研究真菌生物学的几个方面(致病性、菌丝生长、针孔菌的产生、对杀菌剂的敏感性)及其与//的相互作用。antonii。该研究于1999年4月至2000年3月在茂物香料和药用作物病虫害研究所的实验室和温室进行。从腰果叶中直接分离到/' deseminata。以腰果为研究对象,对其病原菌进行了致病性试验。在琼脂培养基上进行了体外真菌生长和杀真菌效果的研究。通过将真菌和昆虫单独或联合接种于腰果幼苗上,研究了该真菌和昆虫之间的相互作用。结果表明,血孢假单胞菌为黑色球形针囊,分生孢子长圆形,有5个细胞,最外层细胞有3个鞭毛虫。只有在600勒克斯半透明灯连续照射的培养基上才能产生刺孢菌。不同分离株对腰果幼苗的致病力试验表明,不同分离株可单独或合并成较大的叶斑。50%百虫腈(1 ppm)和70%乌硫帕酸甲酯(10 ppm)复合接种对真菌菌丝生长均有抑制作用。与单独接种相比,Antonii导致MOE严重(毁灭性)死背。
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