Production and optimization of pterin deaminase from cyanide utilizing bacterium Bacillus cereus AM12

Abstract

In a search of new-fangled antitumor chemotherapeutic agent, the studies are elucidating the enzymes, contributing to the metabolic reprogramming and their potential as therapeutic targets. This study was an endeavor to explore the potential of enzyme pterin deaminase from cyanide degraders. In preliminary and secondary screening, potent cyanide degraders were selected and estimated for pterin deaminase activity. The intracellular crude extract containing enzyme activity was found to be higher in potential strain Bacillus cereus AM12. The optimization conditions for the production of pterin deaminase from this selected strain was studied with the help of Box–Behnken design. The determination coefficient of the model (0.91) was highly significant and indicative of goodness of fit. This design found to be ideal for the selected parameters, such as temperature of 35 °C, pH 8, 0.005 M of pterin and 20% of glucose which yielded maximum production of pterin deaminase (9.99 U/ml). This study might make a major breakthrough in the field of medicine as well as in pharmaceutical compositions when the biological significance of this distinct enzyme is proven.