Pharmacokinetics Of Adriamycin After Intravenous Administration In Rat.

Abstract

Pharmacokinetics of adriamycin in the rat testis, heart and plasma was Investigated using male Wister rats. The control group received 0. 9%saline Given I.V. One group of adriamycin treated rats received 20mg/kg adriamycin administered intravenously. The Second group received1mg/kg adriamycin also given I.V. Thereafter, the animals weresacrificed 1,3,12 and 24 hours and the testes, and hearts were removed.The plasma was recovered from the blood. Using fluorimetric method, the amount of adriamycin in the tissues and plasma was quantitated. The Half life of the drug in the tissues and plasma was calculated from the Graph of log adriamycin concentration (ug/g) vs. time. The pattern of distribution in the heart and plasma at the two doses used in the pharmacokinetic studies revealed an exponential fall in drug concentration with time. In the testis on the other hand, after the initial drop on the level of the drug in this organ, the level of the drug was elevated with time. This ability of the testis to accumulate adriamycin renders it highly susceptible to the lethal effects of adriamycin And thus accounts for the observed drug induced testicular toxicity.