Optimization and effect of dairy industrial waste as media components in the production of hyaluronic acid by Streptococcus thermophilus

N. Mohan, Rengesh Balakrishnan, S. Sivaprakasam
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引用次数: 15

Abstract

ABSTRACT Hyaluronic acid (HA) production using a dairy industrial waste is a more cost-efficient strategy than using an expensive synthetic medium. In this study, we investigated the production of HA using Streptococcus thermophilus under shake flask conditions using dairy industrial waste as nutritional supplements, namely whey permeate (WP) and whey protein hydrolysate (WPH). Preliminary screening using Plackett–Burman design exhibited WP, WPH, initial pH, and inoculum size as significant factors influencing HA titer. Response surface methodology design of four factors was formulated at three levels for enhanced production of HA. Shake flask HA fermentation by S. thermophilus was performed under global optimized process conditions and the optimal HA titer (342.93 mg L−1) corroborates with Box–Behnken design prediction. The molecular weight of HA was elucidated as 9.22–9.46 kDa. The ultralow-molecular weight HA reported in this study has a potential role in drug and gene delivery applications.
乳品工业废弃物作为嗜热链球菌生产透明质酸的培养基组分的优化及效果
利用乳制品工业废物生产透明质酸(HA)是一种比使用昂贵的合成介质更具成本效益的策略。在这项研究中,我们研究了嗜热链球菌在摇瓶条件下以乳清渗透物(WP)和乳清蛋白水解物(WPH)为营养补充剂生产透明质酸。采用Plackett-Burman设计的初步筛选显示,WP、WPH、初始pH和接种量是影响血凝素滴度的显著因素。为提高HA产量,在三个水平上制定了四因素响应面法设计。在全局优化的工艺条件下,嗜热链球菌摇瓶发酵HA,最佳HA滴度(342.93 mg L−1)与Box-Behnken设计预测相符。HA的分子量为9.22 ~ 9.46 kDa。本研究报道的超低分子量HA在药物和基因传递应用中具有潜在的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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