Expressions of regulatory B cells in peripheral blood of patients with Guillain-Barre syndrome

Q4 Medicine

中华神经医学杂志 Pub Date : 2019-11-15 DOI:10.3760/CMA.J.ISSN.1671-8925.2019.11.013

Yan Wang, Chenguang Hao, Yanfang Li, Yugang Zheng, Jian-Xia Ma

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Abstract

Objective To investigate the expressions of regulatory B cells and related cytokines in patients with Guillain-Barre syndrome (GBS). Methods Forty-four patients with GBS admitted to our hospital from October 2018 to June 2019 were enrolled as GBS group; 44 healthy subjects accepted physical examination in our hospital at the same period were selected as control group. Flow cytometry was used to determine the proportions of CD19+CD24hiCD38hi regulatory B cells and CD19+CD24hiCD27+ regulatory B cells in CD19+ lymphocytes in peripheral blood mononuclear cells of the two groups. ELISA was employed to detect the serum contents of interleukin (IL)-10, IL-35 and transforming growth factor (TGF)-β1 of the two groups. Results As compared with those in the control group, the proportions of CD19+CD24hiCD38hi regulatory B cells and CD19+CD24hiCD27+ regulatory B cells in CD19+B lymphocytes of the GBS group were significantly decreased (P 0.05). Conclusion Regulatory B cells play a role in the pathogenesis of GBS through IL-10 and IL-35 cytokine pathways. Key words: Regulatory B cell; Guillain-Barre syndrome; Cytokine; Interleukin

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格林-巴利综合征患者外周血调节性B细胞的表达

目的探讨吉兰-巴雷综合征(GBS)患者调节性B细胞及相关细胞因子的表达。方法2018年10月至2019年6月在我院住院的44例GBS患者作为GBS组;选取同期在我院接受体检的健康受试者44例作为对照组。采用流式细胞术测定两组外周血单核细胞CD19+淋巴细胞中CD19+CD24hiCD38hi调节性B细胞和CD19+CD24hiCD27+调节性B细胞的比例。采用ELISA法检测两组患者血清中白细胞介素(IL)-10、IL-35及转化生长因子(TGF)-β1的含量。结果与对照组相比，GBS组CD19+B淋巴细胞中CD19+CD24hiCD38hi调节性B细胞和CD19+CD24hiCD27+调节性B细胞比例显著降低(P < 0.05)。结论调节性B细胞通过IL-10和IL-35细胞因子途径参与GBS的发病。关键词:调节性B细胞;格林-巴利综合征;细胞因子;白介素

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