W. Nur Sabrina , A.P. Nurul Hidayah , S. Faridah , M.R. Rashid , Y. Nurul Adibah , I. Zamri
{"title":"Electroimmobilization of Nitrate Reductase into Polypyrrole Films on Screen Printed Carbon Electrode (SPCE) for Amperometric Detection of Nitrate","authors":"W. Nur Sabrina , A.P. Nurul Hidayah , S. Faridah , M.R. Rashid , Y. Nurul Adibah , I. Zamri","doi":"10.1016/j.proche.2016.07.025","DOIUrl":null,"url":null,"abstract":"<div><p>An amperometric nitrate biosensor was successfully constructed by the electroimmobilization of the enzyme nitrate reductase from <em>Aspergillus niger</em> with and without β-NADH, a co-factor, into conducting polypyrrole films on screen printed carbon electrode (SPCE). The best performance was registered by nitrate biosensor developed without the β-NADH electroimmobolize with nitrate reducatse on the SPCE. The formation of the optimum NaR-PPy composite on the SPCE was achieved with an applied potential of 0.9 Vs<sup>-1</sup> for 900s. The optimum amperometric response for nitrate was obtained in 0.1M potassium phosphate buffer, pH 7.5 with an applied potential of -0.1 Vs<sup>-1</sup> and 2mM β-NADH. Sensitivity of the nitrate biosensor was found to be in a linear range of 0.01-0.25mM with a regression coefficient of 0.97 and a relative standard deviation (RSD) of 6.6% (n=5).</p></div>","PeriodicalId":20431,"journal":{"name":"Procedia Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proche.2016.07.025","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Procedia Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S187661961630033X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
An amperometric nitrate biosensor was successfully constructed by the electroimmobilization of the enzyme nitrate reductase from Aspergillus niger with and without β-NADH, a co-factor, into conducting polypyrrole films on screen printed carbon electrode (SPCE). The best performance was registered by nitrate biosensor developed without the β-NADH electroimmobolize with nitrate reducatse on the SPCE. The formation of the optimum NaR-PPy composite on the SPCE was achieved with an applied potential of 0.9 Vs-1 for 900s. The optimum amperometric response for nitrate was obtained in 0.1M potassium phosphate buffer, pH 7.5 with an applied potential of -0.1 Vs-1 and 2mM β-NADH. Sensitivity of the nitrate biosensor was found to be in a linear range of 0.01-0.25mM with a regression coefficient of 0.97 and a relative standard deviation (RSD) of 6.6% (n=5).
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