Empirical Study of Changes of Pre-Menstrual Syndrome Model Rats with Liver-qi Invasion and Liver-qi Depression in Peripheral Blood, Sexual Hormones, Different Encephalic Regions and Accommodate Hormones

Abstract

This work successfully used model rats with Pre-Menstrual Syndrome (PMS) liver-qi invasion and liver-qi depression in the early development days to detect the content levels of estradiol (E2), progesterone (P) and prolactin (PRL) in their blood serums and different encephalic regions, to explore the micro-mechanism of the syndrome of the liver failing to maintain the normal flow of qi. 40 rats with estrous cycles not in accepting time were selected and divided randomly into five groups: the normal control group, PMS liver-qi invasion model group, PMS liver-qi depression model group, PMS liver-qi invasion medication-administered group and PMS liver-qi depression medication-administered group. Emotional stimulation and multiple factors combination were used to prepare the PMS liver-qi invasion model. Chronic stress constraints were used to prepare the PMS liver-qi depression model. Jingqianping granules were administered to rats to interfere with the PMS liver-qi invasion mode, and Jingqianshu granules were administered to rats to interfere with the PMS liver-qi depression mode. Radioimmunity was used to determine the content levels of E2, P and PRL in the blood serum and centrum, and to compare and analyze the differences among the five groups. In the open-field experiment, the crossing scores, rearing scores and open-field experiment total scores of rats in the PMS liver-qi invasion model group increased remarkably (P < 0.05) compared with the scores of the normal control group, while the scores of the PMS liver-qi depression model group decreased remarkably (P < 0.05). The tendency was retrieved remarkably after medications (P < 0.05). The content levels of E2 and P in the blood serum of the PMS liver-qi invasion model group tended to decrease compared with rats in the normal control group. The content levels of E2 and P in the blood serum of rats in the PMS liver-qi depression model group decreased remarkably. The changes of hormones in centrum were opposite to the blood serum. Taken togeterh, the occurrence of PMS may be related with the abnormal fluctuations of sexual hormones and accommodated hormones. Jingqianping and Jingqianshu granules can accommodate these abnormal fluctuations in blood serum and centrum, respectively.