Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats

Abstract

The present work was carried out to studytheprotective effect of 70% methanolic extract of Diospyros lotus L. aerial parts against hepato and nephro-toxicity that was induced bycarbon tetrachloride. In addition, phytochemical screening, total phenolic, total flavonoid contents and proximate analysisof the aerial parts were determined. Methanol (70%) extracts of the aerial parts of Diospyros lotus L. was prepared. Two main groups of rats were assigned which were normal and damaged liver groups that administrated CCl4 (carbon tetra chloride). Each group classified into 4 different groups normalcontrol: was received distilled water, positive control: was administered silymarin (50 mg/kg), and treated groups:were administered Diospyros lotus tested extract at two dose levels (150 and 300 mg/kg) for 15 days. Serum alanine and aspartate aminotransferase activities were determined to evaluate hepatoprptective effect, while uric acid and creatinine levels were measured to study renal function protective effects. The results showed that the alterations induced by carbon tetrachloride were significantly restored by methanol extract of Diospyros lotus (150 and 300 mg/kg) in dose dependant manner. Alkaloids, carbohydrates, tannins, flavonoids, saponins and unsaturated sterols and/or triterpenes were detected as the main active constituents of the methanol extract. It can be concluded that the 70% methanol extract of Diospyros lotus L. aerial parts has a significant protective effect against acute hepato-toxicity and nephro-toxicity induced by CCl4 in ratsso it can be used as a safe hepato-nephro protective drug.