Toll-like receptor homolog TOL-1 regulates Bifidobacterium infantis-elicited longevity and behavior in Caenorhabditis elegans

IF 3.1 4区医学 Q2 Agricultural and Biological Sciences

Bioscience of Microbiota, Food and Health Pub Date : 2019-05-23 DOI:10.12938/bmfh.18-031

Simo Sun, Yasuko Mizuno, T. Komura, Yoshikazu Nishikawa, E. Kage-Nakadai

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引用次数: 10

Abstract

Bifidobacterium infantis, a Gram-positive bacterium, is one of the commonly used probiotics. We previously showed that B. infantis modified host defense systems and extended the lifespan of the nematode Caenorhabditis elegans. In the present study, we showed that the lifespan extension caused by B. infantis was enhanced in animals having a mutation in the tol-1 gene that encodes the sole C. elegans homolog of Toll-like receptors (TLRs). Meanwhile, lifespan increased by other probiotic bacteria, such as Bacillus subtilis or Clostridium butyricum, was not affected in the tol-1 mutant animals. A microarray analysis revealed that the expression of innate immune response-related genes was significantly increased in the tol-1 mutant. Worms with the tol-1 mutation exhibited reduced leaving behavior from the B. infantis lawn, while canonical downstream factors trf-1/TRAF and ikb-1/IκB appeared to not be involved. In conclusion, C. elegans tol-1/TLR regulates B. infantis-induced longevity and also regulates behavior against B. infantis.

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toll样受体同源物toll -1调节婴儿双歧杆菌诱导的秀丽隐杆线虫的寿命和行为

婴儿双歧杆菌是一种革兰氏阳性菌，是常用的益生菌之一。我们之前的研究表明，幼芽胞杆菌可以改变宿主的防御系统，延长秀丽隐杆线虫的寿命。在本研究中，我们发现在编码toll样受体(TLRs)唯一的秀丽隐杆线虫同源基因的toll -1基因突变的动物中，由B.婴儿引起的寿命延长得到增强。同时，其他益生菌，如枯草芽孢杆菌或丁酸梭菌，在tol1突变动物中不受寿命延长的影响。微阵列分析显示，先天免疫反应相关基因的表达在toll -1突变体中显著增加。携带toll -1突变的线虫在草坪上的离开行为减少，而典型的下游因子trf-1/TRAF和ikb-1/ i - κ b似乎没有参与。综上所述，秀丽隐杆线虫的tol1 /TLR调节了B.婴儿诱导的寿命，也调节了对B.婴儿的行为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience of Microbiota, Food and Health Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

5.50

自引率

3.20%

发文量

期刊介绍： Bioscience of Microbiota, Food and Health (BMFH) is a peer-reviewed scientific journal with a specific area of focus: intestinal microbiota of human and animals, lactic acid bacteria (LAB) and food immunology and food function. BMFH contains Full papers, Notes, Reviews and Letters to the editor in all areas dealing with intestinal microbiota, LAB and food immunology and food function. BMFH takes a multidisciplinary approach and focuses on a broad spectrum of issues.