PURIFICATION AND PARTIAL CHARACTERISATION OF AN ANTIFUNGAL BACTERIOCIN FROM Bacillus sp. Sh10 ASSOCIATED WITH MARINE CARPET CLAM (Paphia textile)

F. Shayesteh, Asmat Ahmad, G. Usup
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引用次数: 3

Abstract

This study was conducted to purify and characterize an anti- C. albicans bacteriocin produced by Bacillus sp. Sh10 isolated from marine carpet clam, Paphia textile. Extracellularly produced bacteriocin was purified by 80 % ammonium sulfate precipitation and size exclusive gel chromatography using superdex-200 column chromatographyThe purification steps resulted in 3.68-fold increase in specific activity and recovery of 20.66 % of total activity.  The purity rate and molecular mass of 11 KDa of this compound were determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).  Profile analysis of this bacteriocin by electrospray mass spectrometry (LC/MS/MS) showed 43% protein sequence coverage with a putative uncharacterized protein from Bacillus cereus strain B4264.
海毯蛤(帕菲亚纺织)相关芽孢杆菌sp. Sh10抗菌素的纯化及部分特性研究
本研究对从帕菲亚海毯蛤中分离的芽孢杆菌sp. Sh10产生的抗白色念珠菌素进行了纯化和鉴定。采用80%硫酸铵沉淀法和superdex-200柱凝胶层析纯化细胞外产生的细菌素,比活性提高3.68倍,总活性回收率为20.66%。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)测定了该化合物的纯度和分子量为11 KDa。电喷雾质谱(LC/MS/MS)分析表明,该细菌素与蜡样芽孢杆菌B4264菌株的推定未鉴定蛋白序列覆盖率为43%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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