PURIFICATION AND PARTIAL CHARACTERISATION OF AN ANTIFUNGAL BACTERIOCIN FROM Bacillus sp. Sh10 ASSOCIATED WITH MARINE CARPET CLAM (Paphia textile)

F. Shayesteh, Asmat Ahmad, G. Usup
{"title":"PURIFICATION AND PARTIAL CHARACTERISATION OF AN ANTIFUNGAL BACTERIOCIN FROM Bacillus sp. Sh10 ASSOCIATED WITH MARINE CARPET CLAM (Paphia textile)","authors":"F. Shayesteh, Asmat Ahmad, G. Usup","doi":"10.15414/JMBFS.2513","DOIUrl":null,"url":null,"abstract":"This study was conducted to purify and characterize an anti- C. albicans bacteriocin produced by Bacillus sp. Sh10 isolated from marine carpet clam, Paphia textile. Extracellularly produced bacteriocin was purified by 80 % ammonium sulfate precipitation and size exclusive gel chromatography using superdex-200 column chromatographyThe purification steps resulted in 3.68-fold increase in specific activity and recovery of 20.66 % of total activity.  The purity rate and molecular mass of 11 KDa of this compound were determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).  Profile analysis of this bacteriocin by electrospray mass spectrometry (LC/MS/MS) showed 43% protein sequence coverage with a putative uncharacterized protein from Bacillus cereus strain B4264.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Microbiology, Biotechnology and Food Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15414/JMBFS.2513","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3

Abstract

This study was conducted to purify and characterize an anti- C. albicans bacteriocin produced by Bacillus sp. Sh10 isolated from marine carpet clam, Paphia textile. Extracellularly produced bacteriocin was purified by 80 % ammonium sulfate precipitation and size exclusive gel chromatography using superdex-200 column chromatographyThe purification steps resulted in 3.68-fold increase in specific activity and recovery of 20.66 % of total activity.  The purity rate and molecular mass of 11 KDa of this compound were determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).  Profile analysis of this bacteriocin by electrospray mass spectrometry (LC/MS/MS) showed 43% protein sequence coverage with a putative uncharacterized protein from Bacillus cereus strain B4264.
海毯蛤(帕菲亚纺织)相关芽孢杆菌sp. Sh10抗菌素的纯化及部分特性研究
本研究对从帕菲亚海毯蛤中分离的芽孢杆菌sp. Sh10产生的抗白色念珠菌素进行了纯化和鉴定。采用80%硫酸铵沉淀法和superdex-200柱凝胶层析纯化细胞外产生的细菌素,比活性提高3.68倍,总活性回收率为20.66%。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)测定了该化合物的纯度和分子量为11 KDa。电喷雾质谱(LC/MS/MS)分析表明,该细菌素与蜡样芽孢杆菌B4264菌株的推定未鉴定蛋白序列覆盖率为43%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信