The Protective Effect of Rosmarinic Acid on the Aluminum of Dementia Inducer

Abstract

To examine the protective effect of rosmarinic acid on the aluminum of dementia inducer, cultured C6 glioma cells were treated with various concentrations of aluminum chloride (AlCl 3 ) or rosmarinic acid. The cell viability, electron donating ability (EDA), superoxide dismutase (SOD)-like activity, and inhibitory activity of lipid peroxidation were evaluated for the antioxidant effect of rosmarinic acid. In these cultures, AlCl 3 sowed a cytotoxic effect by decreasing the cell viability in a dose-dependent manner; then, the XTT 50 value was measured at 142.2  M of AlCl 3 after treating the cultured C6 glioma cells with media containing 120 ∼ 160  M AlCl 3 . Therefore, its toxicity was determined as mid-cytotoxic by Borenfreund and Puerner’s toxic criteria; while, vitamin E of antioxidant markedly increased the cell viability on AlCl 3 – induced cytotoxicity in these cultures. This study showed the antioxidant effect of rosmarinic acid via several assays, such as electron donating activity (EDA), superoxide dismutase (SOD)-like activity, and inhibitory activity of lipid peroxidation. From these findings, it is suggested that the oxidative stress is involved in AlCl 3 -induced cytotoxicity, and rosmarinic acid was effective in the protection of AlCl 3 -induced cytotoxicity by antioxidant activity. In conclusion, natural resources, like rosmarinic acid, may be a putative antioxidant agent for the treatment of reactive oxygen species (ROS)-mediated disease, such