Isolasi dan Identifikasi Bakteri Resisten Arsen pada Sedimen di Pesisir Laut Buyat Tahun 2018

Jurnal e-Biomedik Pub Date : 2018-10-10 DOI:10.35790/EBM.6.2.2018.21996

Natalia M. Pantow, B. Kepel, Fatimawali

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Abstract

Abstract: Metals are very important and widely used in many aspects of human life, however, they can cause environmental pollution which is harmful to life. Arsenic is a very toxic metal that can cause kidney damage and is responsible to various chronic health problems, especially cancer. The coastal area of Buyat Sea in North Sulawesi Province has a high level of activity in mining industry. The mining waste results in environmental pollution due to arsenic release, thus triggers the occurence of arsenic-resistant bacteria. This study was aimed to determine whether there were arsenic-resistant bacteria in soil sediment originated from the coastal area of Buyat Sea and to identify the types of arsenic-resistant bacteria. This was a descriptive explorative study. Samples of soil sediment were obtained from one point on the coastal area of Buyat Sea. The results showed that there were 10 isolates of arsenic-resistant bacteria. There were five genera of bacteria that were resistant to arsenic, namely Escherichia, Klebsiella, Hafnia, Staphylococcus, and Bacillus. Conclusion: In the coastal area of Buyat Sea, five genera of arsenic-resistant bacteria were identified, as follows: Escherichia, Klebsiella, Hafnia, Staphylococcus, and Bacillus.Keywords: arsenic, sediment, arsenic-resistant bacteria Abstrak: Logam sangat penting dalam berbagai aspek kehidupan manusia walaupun dapat mencemari lingkungan dan berbahaya bagi kehidupan. Arsen merupakan logam berat yang sangat bersifat toksik dan dapat merusak ginjal serta bertanggung jawab terhadap berbagai gangguan kesehatan kronis, termasuk kanker. Daerah pesisir Buyat laut di Provinsi Sulawesi Utara memiliki tingkat aktivitas yang tinggi dalam bidang industri pertambangan. Limbah pertambangan mengakibatkan terjadinya pencemaran lingkungan oleh karena pelepasan arsen yang dapat memicu terjadinya bakteri resisten arsen. Penelitian ini bertujuan untuk mengetahui apakah terdapat bakteri resisten arsen pada sedimen yang berasal dari daerah pesisir laut Buyat dan untuk mengidentifikasi jenis bakteri resisten arsen yang ditemukan. Jenis penelitian ialah deskriptif eksploratif. Sampel penelitian ialah sedimen tanah di satu titik di pesisir laut Buyat. Hasil penelitian mendapatkan 9 isolat bakteri resisten arsen dan ditemukan lima genus bakteri yang resisten terhadap arsen yaitu Escherichia, Klebsiella, Hafnia, Staphylococcus, dan Bacillus. Simpulan: Di daerah pesisir laut Buyat ditemukan bakteri resisten arsen pada sedimen yaitu genus Escherichia, Klebsiella, Hafnia, Staphylococcus, dan Bacillus.Kata kunci: arsen, sedimen tanah, bakteri

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2018年在Buyat sea沉积的沉积物中分离和抗菌鉴定

摘要:金属在人类生活的许多方面有着重要的作用和广泛的应用，但同时也会造成环境污染，危害生命。砷是一种非常有毒的金属，会导致肾脏损伤，并导致各种慢性健康问题，尤其是癌症。北苏拉威西省布亚特海沿岸地区的采矿业活动水平很高。采矿废弃物由于砷的释放造成环境污染，从而引发抗砷细菌的发生。本研究旨在确定布亚特海沿岸地区土壤沉积物中是否存在抗砷细菌，并对其种类进行鉴定。这是一项描述性探索性研究。在布亚特海沿岸的一个地点采集了土壤沉积物样本。结果显示，共分离出10株耐砷菌。对砷有抗性的细菌有5属，分别是埃希氏菌、克雷伯氏菌、哈夫尼亚菌、葡萄球菌和芽孢杆菌。结论:在布亚特海沿岸地区鉴定出5属耐砷细菌，分别为埃希氏菌、克雷伯氏菌、哈夫尼亚菌、葡萄球菌和芽孢杆菌。关键词:砷，沉积物，抗砷细菌摘要:Logam sangat penting dalam berbagai ask kehidupan manusia walaupun dapat mencemari lingkungan dan berbahaya bagi kehidupan。这是一种非常令人兴奋的感觉，它是一种非常令人兴奋的感觉，它是一种非常令人兴奋的感觉。乌拉威西省省长布雅特•布雅特表示，苏拉威西省的经济活动将持续下去。林巴pertambangan mengakibatkan terjadinya penemaran lingkungan oleh karena pelepasan arsen yang dapat memicu terjadinya bakteri抵抗arsen。Penelitian ini bertujuan untuk mengetahui apakah terdapat bakteri resistance arsen parpa sedimen yang berasal dari daerah perisir laut Buyat danuntuk mengidentifikasi bakteri resistance arsen yang ditemukan。Jenis penelitian的翻译是:样品中含有大量的化学成分，这些成分都是由化学成分和化学成分组成的。Hasil penelitian mendapatkan 9分离出耐巴氏杆菌arsen和迪特穆坎利马属巴氏杆菌yang耐巴氏杆菌arsen、克雷伯氏菌、哈夫尼亚菌、葡萄球菌、丹芽孢杆菌。Simpulan: didaerah pesisir laut Buyat ditemukan bakteri resistant arsen paada sedimen yitu属埃希氏菌，克雷伯氏菌，Hafnia，葡萄球菌，丹芽孢杆菌。Kata kunci: arsen, sedimen tanah, bakteri

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Jurnal e-Biomedik

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