Biofilm formation, antibiotic resistance, and genome sequencing of a unique isolate Salmonella Typhimurium M3

Lijuan Yuan, Yang Liu, Cao-wei Chen, Zhenquan Yang, X. Jiao
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引用次数: 3

Abstract

Salmonella Typhimurium is a zoonotic bacterium that can cause salmonellosis, and the major concerns of S. Typhimurium for the food industry are its ability to obtain multidrug resistance and form biofilms on food-contact surfaces. In the current study, the antimicrobial resistance of a strong biofilm former S. Typhimurium M3 was assessed by the diffusion method. Genome sequencing was also applied to obtain the genes related to antibiotic resistance, and biofilm formation of S. Typhimurium M3. Biofilm-forming capacity of S. Typhimurium M3 was found to be strain dependent, and a high number of isolates were strong biofilm formers. The high biofilm-forming isolate S. Typhimurium M3 was resistant to oxacillin, lincomycin, rifampicin, tetracycline, and clindamycin, with the MIC values of 512 μg/mL, 32 μg/mL, 16 μg/mL, 64 μg/mL, and 64 μg/mL, respectively. Genomic annotation of S. Typhimurium M3 showed the presence of genes involved in cellulose biosynthesis, curli production, fimbriae biosynthesis, flagellar assemble, quorum sensing, chemotaxis, and some transcriptional regulators. Antibiotic efflux conferring antibiotic resistance genes, antibiotic inactivation genes, and antibiotic target alteration genes were also identified. The results expand scientific understanding on how Salmonella isolates with high biofilm-forming capacity and multidrug resistance survive in stressful conditions in the industry.
鼠伤寒沙门氏菌M3的生物膜形成、抗生素耐药性和基因组测序
鼠伤寒沙门氏菌是一种可引起沙门氏菌病的人畜共患性细菌,食品工业对鼠伤寒沙门氏菌的主要担忧是其获得多重耐药性和在食品接触表面形成生物膜的能力。本研究采用扩散法对强生物膜原鼠伤寒沙门氏菌M3进行耐药性评价。利用基因组测序获得鼠伤寒沙门氏菌M3的抗生素耐药及生物膜形成相关基因。鼠伤寒沙门氏菌M3的生物膜形成能力与菌株有关,且大量分离株具有较强的生物膜形成能力。高生物成膜性鼠伤寒沙门氏菌M3对奥西林、林可霉素、利福平、四环素和克林霉素耐药,MIC值分别为512 μg/mL、32 μg/mL、16 μg/mL、64 μg/mL和64 μg/mL。鼠伤寒沙门氏菌M3的基因组注释显示存在纤维素生物合成、卷曲体产生、菌毛生物合成、鞭毛组装、群体感应、趋化性和一些转录调控因子等相关基因。抗生素外排导致抗生素耐药基因、抗生素失活基因和抗生素靶改变基因也被确定。研究结果扩大了对沙门氏菌高生物膜形成能力和耐多药的分离株如何在工业压力条件下生存的科学认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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