Synthesis of Chitosan-Co-Lactic Acid Nanoparticles and their Potential as a Carrier of Anticancer Drug

Abstract

This study aims to design some medicinally important biopolymer based Nano carriers. The study undertakes synthesis, characterization of Chitosan-co-lactic acid nanoparticles and optimization of swelling ratio. The Chitosan-co-lactic acid nanoparticles were prepared by the shaking of lactic acid emulsion in Chitosan solution by emulsion crosslinking method and characterised by transmission electron microscope (TEM) analysis (size), and Fourier transform infrared spectra (FTIR) (structural) and SEM (morphology). The particles were dispersed in a phosphate buffer saline (PBS) and the swelling response of these nanoparticles was studied as a function of chemical composition, pH and temperature of swelling medium. To determine the encapsulation efficiency, a centrifugation method was applied. Also, the assessment of biocompatibility has been made on the basis of two in-vitro test which are BSA adsorption test and haemolysis assay. Our result revealed that equilibrium swelling was influenced by the composition of nanoparticles and the swelling medium. These observations and assessment indicate that these nanoparticles have good capability for use in drug delivery and suitable for biomedical application.