The SNP rs3746444 within mir-499a is associated with breast cancer risk in Iranian population

Journal of Cellular Immunotherapy Pub Date : 2016-11-01 DOI:10.1016/j.jocit.2016.08.003

Shahrzad Kabirizadeh , Mansoureh Azadeh , Mahboubeh Mirhosseini , Kamran Ghaedi , Hamzeh Mesrian Tanha

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引用次数: 16

Abstract

Objective

Our study aimed to evaluate the possible association between hsa-mir-499a (rs3746444 A>G) polymorphism and susceptibility to breast cancer in an Isfahanian population.

Materials & methods

In this case–control study we enrolled 91 healthy subjects and patients with breast cancer. Allele-specific primer PCR was applied for genotyping the SNP.

Results

Our study showed that the hsa-mir-499a rs3746444 G allele increased the risk of breast cancer regarding to allele frequency differences (OR: 1.922; 95% CI: 1.064–3.470; p = 0.02952) and Armitage's trend test (OR: 1.722; p = 0.04732) in comparison with the A allele. In addition, an in silico attempt to find functional consequences of A>G substation suggested that the G allele may decrease hsa-mir-499a stability based on calculated free energy differences between A and G alleles.

Conclusion

Our findings illustrated that the mir-499a rs3746444 G polymorphism is associated with higher risk of developing breast cancer in Isfahanian population.

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mir-499a中的SNP rs3746444与伊朗人群乳腺癌风险相关

目的本研究旨在评估伊斯法罕人群中hsa-mir-499a (rs3746444 A>G)多态性与乳腺癌易感性之间的可能关联。材料,技术的这个病例对照研究,我们招收了91名健康受试者和乳腺癌患者。采用等位基因特异性引物PCR对SNP进行基因分型。结果我们的研究表明，在等位基因频率差异方面，hsa-mir-499a rs3746444 G等位基因增加了乳腺癌的风险(OR: 1.922;95% ci: 1.064-3.470;p = 0.02952)和阿米蒂奇的趋势检验(OR: 1.722;p = 0.04732)。此外，一项寻找A>G变位的功能后果的计算机尝试表明，基于计算的A和G等位基因之间的自由能差，G等位基因可能会降低hsa-mir-499a的稳定性。结论我们的研究结果表明，mir-499a rs3746444 G多态性与伊斯法罕人乳腺癌的高风险相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Cellular Immunotherapy

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