Purification of Z-Ligustilide by AgNO_3-Impregnated Silica Gel

Abstract

OBJECTIVE To investigate the application of AgNO 3 -impregnated silica gel in a format of column chromatography for the isolation and purification of Z-ligustilide from the essential oil of Ligusticum chuanxiong Hort.. METHODS AgNO 3 (2%) was impregnated to the common silica gel to generate a new column chromatographic media. The essential oil was eluted with the mobile phase (petroleum ether: acetoacetate=98.5 1.5), and detected by TLC and UV-Vis spectrometer. The product Z-ligustilide was characterized by GC-MS and HPLC techniques. RESULTS Z-Ligustilide with the purity of 98.87% was effectively purified. CONCLUSION The present study describes a method for rapid isolation of highly pure Z-ligustilide at low cost, allowing future scale-up for modern industrial production. KEY WORDS: Z-ligustilide; AgNO 3 -silica gel; isolation and purification; GC-MS; HPLC Tel: (852)64870314 E-mail: sxucerycery@126.com * Tel: (852)62265496 E-mail: jrong@hku.hk (Ligusticum chuanxiong Hort.) !"#$% &'()* +,-./0 1 23456 78 9 : ;<=>?@ABCD AE FG@"H IJKLMNOPQR [1-2] #ST +,UV WXYZ[\]A COX-2 )^_`a PGE-2 bc d efghij\hk^lm " [3-4] # nopq +, rst uvM -H I23E wxyZ[z{!UVWXY ROSbc>\h MAPK NF-κB AP-1|}V~ F€‚ƒ „ [5] …VW PI3K> Nrf2V~ †E‡EQˆv z{‰Š€‹Œ„ [6-7] #n opq Ž 1#'()* nopq;†‚ ‘’“”TU•:–—u78˜™Qš› HI œL Q [8] #žŸ nopq ‚X¡>¢ £‚¤ „ U K?@¥¦§ ̈ ©Jaf «¬h­®![9] #̄ °±23 † +, ́ GC-MSMμ ¶$· ̧ 1 80o»1⁄4vM 1⁄23⁄4 ¿ÀÁÂÀÁÃÀÄÅpqÁnopqÁ p q A ® [10] #ST +, nopq> pq A ÆÇÃÀÄÅpq ŽÈÉ ˆvÊ Ë ÌÍÎÏÐMÑJÒÓnopq ÔÕ#