Synthesis, characterization and antimicrobial activity of chitosan/Ag nanocomposite using Escherichia coli D8

Abstract

The present study provided a green approach for chitosan/silver nanocomposite (CS/AgNC) synthesis. CS/AgNC were synthesized by a biological method using the supernatant of Escherichia coli D8 (MF062579) strain in the presence of sun light. The absorbance spectra of particle solutions have been characterized by UV-Vis spectrophotometry, Fourier transform-infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM). The obtained CS/AgNC showed absorption peak at 446 nm with an average particle size of ~21-31 nm. Moreover, CS/AgNC showed a potent antimicrobial activity against Candida albicans , Staphylococcus aureus and E. coli .