NATURAL FOOD PRESERVATION SYSTEM AS ALLYL ISOTHIOCYANATE AND EDIBLE BROWN SEAWEED LAMINARIA JAPONICA AGAINST SELECTED ANAEROBIC FOODBORNE BACTERIA: CLOSTRIDIUM PERFRINGENS AND CAMPYLOBACTER JEJUNI

Reham A. Elfayoumy
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引用次数: 3

Abstract

Allyl isothiocyanate (AITC) is basic compound in mustard essential oil and found in some other species of Brassicaceae. It has antimicrobial properties against wide range of foodborne pathogens. Its food industry use as a natural preservative is limited by its quick evaporation, taste pungency, and weak water solubility. The minimum inhibitory concentration (MIC) for AITC was determined by Microdilution assay, respectively. According to the results of the same assay, bactericidal effect was recorded against both tested bacteria. By using vapor and solution deposition ways into Laminaria japonica de-oiled powder, the antimicrobial effect of AITC vapor was demonstrated against the selected bacteria; up to 91% of colony deactivation occurred within 72 h for C. perfringens, while 97% for C. jejuni, the decay behavior of both solution and vapor -deposited were parallel. At 72 h, the de-oiled L. japonica powder was adsorbed AITC twice that of the raw powder approaching 99% theoretical maximum amount. The continued antimicrobial activity of AITC after contact proves that AITC + de oiled L. japonica powder would be used as natural system for preserve food.
以异硫氰酸烯丙酯和日本褐藻为原料的天然食品保鲜体系,对产气荚膜梭菌和空肠弯曲杆菌等厌氧食源性细菌的抑制作用
异硫氰酸烯丙酯(AITC)是芥菜精油中的基础化合物,在其他芸苔科植物中也有发现。它具有抗多种食源性病原体的抗菌特性。它作为天然防腐剂在食品工业中的应用受到蒸发快、味道辛辣、水溶性弱的限制。采用微量稀释法测定AITC的最低抑菌浓度(MIC)。根据同一试验的结果,记录了对两种被试细菌的杀菌效果。采用气相沉积法和溶液沉积法对海带脱油粉进行了抑菌实验,验证了AITC气相对所选细菌的抑菌效果;多达91%的殖民地失活发生在72 h c . perfringens而97% c .空肠的衰减行为解决方案和蒸汽沉积是平行的。在72 h时,脱油粳米粉的AITC吸附量是原料粉的2倍,接近理论最大值的99%。AITC与脱油粳米粉接触后的持续抑菌活性证明了AITC +脱油粳米粉可作为天然的保鲜体系。
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