"Anthelmintic Activity in Vitro of Ceratotheca Sesamoides Endl and Striga Hermonthica (Delile) Benth Aqueous Extracts on Haemonchus Contortus Adult Worms"

Abstract

Citation: Amadou Dicko, Almamy Konate, Arnaud S.R. Tapsoba, Kisito Tindano, Moumouni Sanou, et al., Anthelmintic Activity in Vitro of Ceratotheca Sesamoides Endl and Striga Hermonthica (Delile) Benth Aqueous Extracts on Haemonchus Contortus Adult Worms. Biomed J Sci & Tech Res 40(2)-2021. BJSTR. MS.ID.006414. In order to contribute for alternatives solutions to the chemical fight against NGIs, we carried out in vitro anthelmintic activity test of C. sesamoïdes, and of S. hermonthica aqueous extracts on adult worms of H. contortus. Three concentrations of each extract has be done; 100mg / ml, 50mg / ml and 25mg / ml. A negative control (PBS 1X) and a positive control levamisole at 2.5 mg / ml were constituted. The test was performed in three replicates with three replicas for each concentration per replicate. Adult worms were contacted with each concentration and then incubated in petri dishes (60X15cm) at 27 °C for 20 hours. The results showed a mortality rate of 79.86% at the concentration of 100mg / ml of C. sesamoids and 22.13% at the same concentration of the aqueous extract of S. hermonthica after 20 hours. Statistical analysis shows high significance P (<0,05). The LC (50) obtained were 78.74mg / ml and 212.13mg / ml respectively for C. sesamoides and S. hermonthica