The impact of pH value in the Phenolic content and Antioxidant potential of Medicinal plant extract

Asian Journal of Research in Chemistry Pub Date : 2022-10-01 DOI:10.52711/0974-4150.2022.00059

Berra Djamila, Dia Ouahida, Laouini Salah Eddine, Menaceur Souheila

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引用次数: 1

Abstract

In this research, the aim was to investigate the influence of pH value in the extraction medium on the phytochemical composition and antioxidant activity of Artemisia herba-alba species used in traditional medicine. For this reason, two analytical methods were used (UV-visible spectroscopy, HPLC). Antioxidant properties of the extracts at different pH were estimated by two tests: total antioxidant capacity (TAC) and DPPH free radical scavenging capacity. The results of the evaluation of phenolic compounds showed that the aqueous extracts in a weak acid medium had a high percentage of phenol content, and the highest value for total phenol content was 90.759±1.267mg GAE/g. Whereas, the HPLC analysis indicated the presence of most of the standard phenolic compounds in the majority of the extracts. For antioxidant activity, the best antioxidants reported in extract at pH=6, with a semi-maximal inhibitory concentration (0.151±0,032mg/ml) and a total antioxidant capacity (TAC) of 66.840±0.241mg EAA/g, which indicates that it is effective antioxidant for therapeutic applications.

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pH值对药用植物提取物酚类物质含量及抗氧化能力的影响

本研究旨在探讨提取培养基pH值对传统药用白蒿植物化学成分及抗氧化活性的影响。为此，采用了两种分析方法(紫外可见光谱法和高效液相色谱法)。通过总抗氧化能力(TAC)和清除DPPH自由基能力两项试验，对不同pH下提取物的抗氧化性能进行了评价。酚类化合物评价结果表明，在弱酸介质中提取的水提物酚含量较高，总酚含量最高为90.759±1.267mg GAE/g。而高效液相色谱分析表明，大多数提取物中存在大多数标准酚类化合物。在抗氧化活性方面，pH=6时提取物的抗氧化活性最好，半最大抑制浓度为0.151±0.032 mg/ml，总抗氧化能力(TAC)为66.840±0.241mg EAA/g，是一种有效的抗氧化剂。

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Asian Journal of Research in Chemistry

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